EVALUATION OF ANTI-THYROID ACTIVITY OF FICUS RACEMOSA LINN BARK IN MALE RATS
Keywords:Thyroxine, Hyperthyroidism, Ficus racemosa, Ethanolic extract
Objective: The present study was formulated in order to evaluate the Antithyroid potential of Ficus racemosa Linn bark in Albino rats.
Methods: Ficus racemosa (L.) (Family: Moraceae), is used in the mythical system for abortion, diabetes and urinary disorders. The genus of Ficus exhibited the presence of tyrosine which is responsible for the formation of T3 and T4 hormones. Hyperthyroidism was induced in experimental rats by administering Thyroxine (600Âµ/kg/ml) orally for 14 days. Hyper thyroid male albino rats weighing 150-200 g were treated with oral doses of 250 mg/kg, 350 mg/kg and 450 mg/kg of Ficus racemosa ethanolic extract for a period of 21 days. Methimazole 0.04% w/v for 21 days served as the standard.
Results: In this study, morphological assessment demonstrated that thyroxine treated gathering demonstrates increased levels of Triiodo-L-thyronine and L-thyroxine. Simultaneous administration of ethanolic extracts of Ficus racemosa bark lowered the increased levels. The decrease in the levels of T3 and T4 by the extracts was compared with the reference drug Methimazole.
Conclusion: The Antithyroid activity is evident from the decreased T3 and T4 levels. It can be said that Ficus racemosa significantly by virtue of the presence of tyrosine, which may be useful for further molecular studies to determine the exact mechanism for its Antithyroid activity.
Agyare C. An ethnopharmacological survey and in vitro confirmation of ethnopharmacological use of medicinal plants used for wound healing in bosomtwi-atwima-kwanwoma area, Ghana. J Ethnopharmacol 2009;125(3):393-403.
Rivlin RS. Historical perspective on the use of garlic. J Nutr 2001;131(3s):951-4.
"100,000 Older People Missing Thyroid Treatmentâ€”Study". Available from: http://www.bbc.co.uk/news/health-12252813
Khan A. Thyroid disorders, Etiology and prevalence. J Med Sci 2002;2:89-94.
Be Thyroid Aware; http://www.thyroidweek.com/en/be-thyroid-aware/
Jahan IA, Nahar N, Mosishuzzaman M. Hypoglycaemic and antioxidant activities of Ficus racemosa linn fruits. Nat. Prod. Res 2008;23:399-408.
Mandal SC, Maity TK, Das J, Saba BP, Pal M. Antiinflammatory evaluation of Ficus racemosa linn leaf extract. J Ethnopharmacol 2000;72:87-92.
Baslas RK, Agha R. Isolation of hypoglycaemic principle from the bark of Ficus glomerata Roxb. Himalayan Chem Pharm Bull 1985;2:13-4.
Winterhoff H, Sourgens H, Kemper FH. Antihormonal effects of plant extracts/pharmacodynamic effects of Lithospermum officinale on the thyroid gland of rats; comparison with the effects of iodide. Hormone Metab Res 1983;15:503-7.
Kokate CK. A textbook of pharmacognosy 45th ed. Vallabh Prakashan: New Delhi; 2010.
Khandelwal KR. Practical pharmacognosy techniques and experiments, Second edition. Nirali Prakashan, Pune; 2000. p. 149-56.
Trease GE, Evans WC. A textbook of pharmacognosy, 13th edition. Bailliere Tindall Ltd: London; 1989.
Kulkarni SK. Handbook of experimental pharmacology, second ed. Vallabh Prakashan, Mumbai; 1993.
Vasundhara S, Dharamveer, Rajiv G, Shubhini AS. Ficus carica leaf extract in regulation of thyroidism using ELISA technique. Asian J Pharm Clin Res 2012;5(2):44-8.
Shuurs AHWM, Van WBK. Review. Enzyme Immunoassay. Clin Chem 1977;81:1.
Soos M, Siddle K. Enzymes immuno assay for the quantitative determination of TSH. J Immunol Methods 1982;51:57-68.
Wybenga DR, Pileggi. Diagnostic reagent kit for the in vitro determination of Cholesterol and HDL cholesterol in serum/plasma. Clin Chem 1970;16:980.
Chakrabarti S, Guria S, Samata I, Das M. Thyroid dysfunction modulates glycol regulatory mechanism in rats. Indian J Exp Biol 2007;45:549-53.