TOTAL POLYPHENOL CONTENT AND ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF THE SRI LANKAN ENDEMIC PLANT GENUS SCHUMACHERIA

Authors

  • Chamara Janaka Bandara University of Peradeniya
  • D Nedra Karunaratne University of Peradeniya
  • Anura Wickramasinghe University of Peradeniya
  • D. S. A Wijesundara University of Peradeniya
  • B. M. Ratnayake Bandara University of Peradeniya
  • Veranja Karunaratne Sri Lanka Institute of Nanotechnology and University of Peradeniya

Keywords:

Schumacheria, Endemic genus, Total polyphenols, Antioxidant, Cytotoxic

Abstract

Objective: Schumacheriais a genus endemic to Sri Lanka distributed in lowland rain forests and mountain rain forests. The total polyphenol content and antioxidant and cytotoxic activities of hexane, dichloromethane and methanol extracts of stem-bark, root-bark, leaves and flowers of the three representative plant speciesS. castaneifolia, S. alnifolia and S. angustifolia belonging to the endemic genus Schumacheriaare reported.

Methods: Antioxidant activity was determined using the DPPH (1,1-diphenyl-2-picrylhydrazine) radical scavenging assay. The total polyphenol content, expressed as the gallic acid equivalent, was determined using theFolin-Ciocaltue method and the cytotoxic activity was determined using the brine shrimp (Artemiasalina) assay.

Results: The methanol extract of S. castaneifolia flowers (IC50 = 6.8 ± 0.1 ppm)and the methanol extracts of S. alnifolia stem-bark (IC50 =7.1 ± 0.4 ppm) and leaves (IC50 =8.3 ± 0.3 ppm) showed antioxidant activity higher than that of α-tocopherol(IC50 = 10.9 ± 4.3 ppm). The methanol extracts of S. alnifolia stem-bark (69.3 ± 6.9 mg g-1) and leaf (57.7 ± 0.0 mg g-1) extracts showed the highest polyphenol content closely followed by the methanol extracts of S. castaneifolia flowers (71.2 ± 0.6 mg g-1). The highest cytotoxic activity was exhibited by the methanol extract of S. castaneifolia flowers (LC50 = 1.3 ± 0.7 ppm), and all the other extracts showed comparatively less cytotoxic activity.

Conclusion: S. castaneifolia and S. alnifolia exhibit potent bioactivitiesvalidating the ethnomedical claims where the former has been used for oral aphthous.

 

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References

Orange A, Wolseley P, Karunaratne V, Bombuwela K. Two leprarioid lichens new to Sri Lanka. Bibl Lichenol 2001;78:327-33.

Jayalal RGU, Wolseley PA, Gueidan C, Aptroot A, Wijesundara DSA, Karunaratne V. Anziamahaeliyensisand Anziaflavotenuis, two new species from Sri Lanka. Lichenol 2012;44(3):381-9.

Bandara BMR, Fernando IHS, Hewage CM, Karunaratne V, Adikaram NKB, Wijesundara DSA. Antifungal activity of some medicinal plants of Sri Lanka. J Natl Sci Counc Sri Lanka 1989;17(1):1-13.

Bandara BMR, Hewage CM, Jayammane L, Karunaratne V, Bandara KANP, Adikaram NKB, et al. Biological activity of some steam distillates from leaves of ten species of Rutaceous plants. J Natl Sci Counc Sri Lanka 1990;18(l):71.

Hewage CM, Bandara KANP, Karunaratne V, Bandara BMR, Wijesundara DSA. Insecticidal activity of some medicinal plants of Sri Lanka. J Natl Sci Counc Sri Lanka 1997;25(3):141-50.

Hewage CM, Bandara BMRB, Karunaratne V, Wannigama GP, Pinto MRM, Wijesundara DSA. Antibacterial activity of some medicinal plants of Sri Lanka. J Natl Sci Counc Sri Lanka 1998;26(l):27–34.

Bandara BMR, Cortez D, Jayasinghe L, Karunaratne V, Wannigama GP, Sotheeswaran S. Aphorphine alkaloids from Litseagardineriand Actinodaphnespeciosa. Planta Med 1989;55:393.

Bandara BMR, Jayasinghe L, Karunaratne V, Wannigama GP, Bokel M, Kraus W, et al. Isolation of pheanthine from Cycleaburmanai. Planta Med 1990;56:245.

Gunatilaka AAL, Dhanabalasingham B, Karunaratne V, Kikuchi T, Tezuka Y. Studies on terpenoids and steroids. Part 27. Structure of a D: A-Friedo-oleanane Triterpenoid from Salacia reticulataand revision of the structure of kokoonol, kokzeylanol series of triterpenoids. Tetrahedron 1993;49(45):10397.

Dhanabalasingham B, Karunaratne V, Gunatilaka AAL, Tezuka Y, Kikuchi T. Biogenetically important quinonemethides and other constituents of Salacia reticulata. Phytochem 1996;42:1377.

Kathirgamanathar S, Ratnasooriya WD, Baekstrom P, Andersen RJ, Karunaratne V. Chemistry and bioactivity of physciaceae lichens pyxine consocians and Heterodermia leucomelos. Pharm Biol 2006;44(3):217-20.

Karunaratne V, Hoveyda HR, Orvig C. General method for the synthesis of trishydroxamic acids. Tetrahedron Lett 1992;33(14):1827-30.

Ratnayake R, Bandara BMR, Wijesundara DSA, Carr G, Andersen RJ, Karunaratne V. Four butanolides derivatives from Hortonia, a genus endemic to Sri Lanka. J Chem Res 2008;3:134-6.

Ratnayake Bandara BM, Hewage CM, Karunaratne V, Adikaram NKB. Methyl ester of para-coumaric acid: antifungal principle of the rhizome of Costusspeciosus. Planta Med 1988;54(5):477-8.

Somesekaram T, Editor. Arjuna’satlas of Sri Lanka. Arjuna Consulting Company; 1997. p. 36-8.

Dassanayake MD, Editor. A Revised handbook to the flora of Ceylon. AmarindPublishers: New Delhi; 1980-1987. p. 131-2.

MOE. The national red list of Sri Lanka: conservation status of the fauna and flora. Ministry of Environment, Colombo, Sri Lanka; 2012. p. 476.

Gunatilleke IAUN, Gunatilleke CVS. Distribution of endemics in the tree flora of a lowland hill forest in Sri Lanka. Biol Conserv 1984;28:275-85.

Budzianowski J, Budzianowska A. Chromatographic and spectrophotometric analyses of the DPPH free radical scavenging activity of the fractionated extracts from Lamium album L, Lamiumpurpureum L, Viscum album L. Herba Pol 2006;52(1/2):51-7.

Mayer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, Mclaughlin JL. Brine shrimp: a convenient bioassay for active plant constituents. Planta Med 1982;45:31-4.

Ratnayake R, Karunaratne V, Bandara BMR, Kumar V, Macleod JK, Simmonds P. Two new lactones with mosquito larvicidal activity from three Hortoniaspecies. J Nat Prod 2001;61:376–8.

Palmer DM, Kitchin JS. Oxidative damage, skin aging, antioxidants and a novel antioxidant rating system. J Drugs Dermatol 2010;9(1):11-15.

MozetiÄ B, TomažiÄ I, Å kvarÄ A, TrebÅ¡e P. Determination of polyphenols in white grape berries cv. Rebula. Acta Chim Slov 2006;53:58–64.

Published

01-03-2015

How to Cite

Bandara, C. J., D. N. Karunaratne, A. Wickramasinghe, D. S. A. Wijesundara, B. M. R. Bandara, and V. Karunaratne. “TOTAL POLYPHENOL CONTENT AND ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF THE SRI LANKAN ENDEMIC PLANT GENUS SCHUMACHERIA”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 465-7, https://innovareacademics.in/journals/index.php/ijpps/article/view/4308.

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Section

Short Communication(s)