IN VITRO ANTIBACTERIAL ACTIVITY OF LEAF, SEED, ROOT, POD AND FLOWER EXTRACTS OF CAJANUS CAJAN (L.) MILLSP

Authors

  • Mahitha Banala Department of Biotechnology Kakatiya University Warangal
  • Srikanth Kagithoju Department of Biotechnology Kakatiya University Warangal
  • Archana Pamulaparthi Department of Biotechnology Kakatiya University Warangal
  • Chamundeshwari Edara Department of Biotechnology Kakatiya University Warangal
  • Ramaswamy Nanna Department of Biotechnology Kakatiya University Warangal

Keywords:

Cajanus cajan, Phytochemicals, Staphylococcus auereus, Escherichia coli, Antibacterial activity

Abstract

Objective: The aim of this study was to investigate the antibacterial activity of leaf, seed, root, pod and flower extracts of C. cajan with various solvents such as aqueous, acetone, chloroform, ethanol and methanol.

Methods: C. cajan was evaluated against certain pathogenic species of gram negative and gram positive bacteria (Staphylococcus aureus and Escherichia coli) by agar well diffusion method.

Results: It was observed that the methanolic and ethanolic extracts had shown potent antibacterial activity against gram +ve and gram -ve bacterial species. Among the five solvents used, methanolic extracts showed higher activity against S. aureus (ZI of leaf, 25.5±0.08 mm;  seed, 22.6±0.01 mm;  root, 22.1±0.09 mm;  pod, 20.8±0.14 mm and flower, 21.9±0.05 mm). Whereas ethanolic extracts showed higher activity against E. coli of all the solvent extracts used in C. cajan (ZI of leaf, 25.1±0.05 mm;  seed, 22.4±0.12 mm;  root, 21.9±0.04 mm;  pod, 20.7±0.12 mm and flower, 21.5±0.10 mm). The minimum inhibitory concentration of methanolic and ethanolic leaf, seed, root, pod and flower extracts respectively was determined to be ranging between 0.38 and 0.512mg/mL for both the bacterial species.

Conclusion: The results of this study support that the crop species C. cajan had potential antibacterial activity against S. aureus and E. coli and these extracts may be used for production of drugs commercially to treat diseases caused by the respective pathogens.

 

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References

Duke JA. Hand book of legumes of world economic importance. New York: Plenum press; 1981.

Ogunbinu AO, Flamini G, Cioni PL, Adebayo MA, Ogunwande IA. Constituents of Cajanus cajan (L) Millsp, Moringa oleifera Lam, Heliotropium indicum L and Bidens pilosa L from Nigeria. Nat Prod Commun 2009;4(4):573-8.

Brink M, Belay G. Cereals and Pulses PROTA Foundation/Backhuys Publishers Wageningen; 2006. p. 35.

Khare CP. Indian medicinal plants: An illustrated dictionary springer-verlag berlin; 2007. p. 110.

Azoro C. Towards effective integration of herbal medicine practice. Nigerian J Res Prod 2004;3:132-7.

Sofowora A. African medicinal plants: Proceedings of a Conference. University of Ife press, lle-Ife; 1979. p. 24.

Soberon JR, Sgariglia MA, Sampietro DA, Quiroga EN, Vattuone MA. Antibacterial activity of plant extract from Nothwest Argentina. J Appl Microbiol 2007;102:1450-61.

Srikanth K, Vikram G, Shiva Krishna P, Hima Bindhu K, Ramaswamy N. Antibacterial activity of flower extract of Pongamia pinnata Linn-an elite medicinal plant. Int J Pharm Pharm Sci 2012;4:3.

Hema TA, Shiny M, Parvathy J. Antimicrobial activity of leaves of Azima tetracantha against clinical pathogens. Int J Pharm Sci 2012;4(4):317-9.

Entani E, Asai M, Tsujihata S, Tsukamoto Y, Ohta M. Antibacterial action of vinegar against food-born pathogenic bacteria including Escherichia coli 0157:H7. J Food Prot 1998;61(8):953-9.

Rama Swamy N, Mahitha B, Archana P, Archana K, Srikanth K. Evaluation of Phytochemicals and fluorescent analysis of seed and leaf extracts of Cajanus cajan L. Int J Pharm Sci Rev Res 2013;22(1):11-8.

Mahitha B, Archana P, Srikanth K, Ramaswamy N. Pharmacognostical and preliminary phytochemical investigations in stem and root of Cajanus cajan (L) millsp. Res J Biotech 2014;9(7):36-42.

Opera AA, Anasa MA. The antibacterial activity of tea and coffee on selected microorganisms. J Med Lab Sci 1993;3:45-8.

Koneman EW, Allen SD, Janda WN, Schereckenberger PC, Winn WC. The family Streptocococaceae†taxonomy and clinical signifigance. Color Atlas and text book of diagnostic microbiology. 5th Ed. Pa: Lippincott, Philadelphia: Williams and Wilkins Publishers; 1997. p. 581.

National committee for clinical laboratory standards. Performance standards for antimicrobial disk susceptibility tests; approved standard. 7th ed. NCCLS document M2-A7, Wayne, PA; 2000. p. 20.

Janovska D, Kubikova K, Kokoska L. Screening for antimicrobial activity of some medicinal plant species of tradtional Chinese medicine. Czech J Food Sci 2003;21(3):107-10.

Pillai SK, Sinha HC. Statistical methods for biological works. Ram prasad and Sons, Agra; 1968.

Obiorah S, Eze E, Obiorah D, Orji N, Umedum C. Phytochemical and antibacterial studies on the extracts from leaves of Cajanus cajan and Eucalyptus Globulus. Int Conf Environ Chem Biol 2012;49(38):192-7.

Ezeifeka GO, Orji MU, Mbata TI, Patrick AO. Antimicrobial activities of Cajanus cajan, Gracinia kola and Xylopia aethiopica on pathogenic microorganisms. Biotechnol 2004;3(1):41-3.

Majorie MC. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12(4):564-82.

Prathima H, Prathima M. Antibacterial activity of various leaf extracts of Cajanus cajan L. Bioscan 2011;6(1):111-4.

Jigna P, Sumitra C. In vitro antimicrobial activities of extracts of Launaea procumbens Roxb. (Labiateae), Vitis vinifera L. (Vitaceae) and Cyperus rotundus L. (Cyperaceae). Afr J Biomed Res 2006;9(2):89-93.

Dougharia JH, Manzara S. In vitro antibacterial activity of crude leaf extracts of Mangifera indica Linn. Afr J Microbiol Res 2008;2:67-72.

Akunyilli AN, Houghton DJ, Romana. Antibacterial activities of the stem bark of Kigelia pinnata. J Ethanopharmacol 1991;2:173-7.

Published

01-03-2015

How to Cite

Banala, M., S. Kagithoju, A. Pamulaparthi, C. Edara, and R. Nanna. “IN VITRO ANTIBACTERIAL ACTIVITY OF LEAF, SEED, ROOT, POD AND FLOWER EXTRACTS OF CAJANUS CAJAN (L.) MILLSP”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 265-8, https://innovareacademics.in/journals/index.php/ijpps/article/view/4318.

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