• A Brinda Devi Sathyabama University
  • C. Valli Nachiyar Department of Biotechnology Sathyabama University Sholinganallur Chennai 600 119 Tamil Nadu
  • T. Kaviyarasi Sathyabama University
  • Antony V. Samrot Sathyabama University


Bacillus cereus, Growth, Biodegradable PHB, Characterization, Nanoparticles


Objectives: To characterize the Polyhydroxybutyrate (PHB) produced from Bacillus cereus using various instrumental methods and reduce them to the nanoscale which can be used as drug carrier

Methods: The isolated bacterium was identified by 16S rDNA analysis. PHB produced by the bacterium was extracted by a process using boiling chloroform followed by methanol which selectively precipitates PHA. The polymer produced was analyzed using various instrumental techniques like FTIR, GC-MS, 1Hand 13C NMR, XRD and FESEM. This biogenic PHB has been reduced to nanospheres which were analyzed by FESEM.

Results: A bacterium with the ability to produced PHB was isolated from the cloth used to smear oil on pan cake pan which was identified as Bacillus cereus by 16S rDNA analysis. The organism was capable of accumulating 1.19 g L-1 of PHA corresponding to 49.7% of its dry weight after 48 h of incubation. The polymer produced was analyzed using various instrumental techniques which identified the polymer as PHB.

Conclusion: The organism seems to be a potential candidate for the biogenic synthesis of PHB which can find application as drug carrier.



Download data is not yet available.

Author Biography

C. Valli Nachiyar, Department of Biotechnology Sathyabama University Sholinganallur Chennai 600 119 Tamil Nadu

Professor and Head

Department of Biotechnology
Sathyabama University
Chennai 600 119
Tamil Nadu


Chen GQ, Wu Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomater 2005;26:6565-78.

Lenz RW, Gross RA, Brandl H, Fuller RC. Poly (9-hydroxyalkanoates): natural biocompatible and biodegradable polyesters produced by bacteria. Chin J Polym Sci 1989;7:289-98.

Meenatchi Sundaram P. Mixed Plastics Film Laminate Recycling–Need of the Hour. Available from: URL: http:// 20Film%20Lamin ate %20 Recycling%20%E2% 80%93% 20Need%20of%20the%20Hour.pdf

Chen GQ. Plastics from Bacteria: Natural functions and applications. microbiology monographs, Berlin Heidelberg: © Springer-Verlag; 2010.

Koller M, Salerno A, Muhr A, Reiterer A, Braunegg G. Polyhydroxyalkanoates: biodegradable polymers and plastics from renewable resources. Mater Technol 2012;46:23-30.

Byron D. Polymer synthesis by microorganisms: technology and economics. Trends Biotechnol 1987;5:246–50.

Dawes EA, Senior PJ. The role and regulation of energy reserve polymers in microorganisms. Adv Microb Physiol 1973;10:135-266.

Pringsheim EG, Wiessner W. Minimum requirements for heterotrophic growth and reserve substance in Beggiatoa. Nat 1963;197:02.

Kannan LV, Rehacek Z. Formation of Poly-β-hydroxybutyrate by Acinomycetes. Indian J Biochem 1970;7:126-9.

Carr NG. The Occurrence of Poly-beta-hydroxybutyrate in the blue-green alga Chlorogloea fritschii. Biochim Biophys ACTA 1996;120:308-10.

Yilmaz M, Soran H, Beyatli Y. Determination of poly-β-hydroxybutyrate (PHB) production by some Bacillus spp. World J Microbiol Biotechnol 2005;21:565-6.

Wu Q, Huang HH, Hu GH, Chen JC, Ho KP, Chen GQ. Production of poly-3-hydroxybutyrate by Bacillus sp. JMa5 cultivated in molasses media. Anton Leeuw Int J G 2001;80:111-8.

Brinda Devi A, Valli Nachiyar C, Kaviyarasi T. Bacillus cereus mediated synthesis of green plastics-Polyhydroxybutyrate. J Pure Appl Microbiol 2014;8:4143-7.

Woese CR. Bacterial evolution. Microbiol Rev 1987;57:221-71.

Garrity G. AAVV: Bergey’s manual of systemic bacteriology. London: Springer; 2005.

Kessler B, Weusthuis R, Witholt B, Eggink G. Production of microbial polyesters: fermentation and downstream processes. Adv Biochem Eng 2001;71:159-82.

Lee EY, Choi CY. Structural identification of polyhydroxyalkanoic acid (PHA) containing 4-hydroxyalkanoic acids by gas chromatography-mass spectrometry (GC-MS) and its application to bacteria screening. Biotechnol Tech 1997;11:167-71.

Xiong YC, Yao YC, Zhan XY, Chen GQ. Application of polyhydroxyalkanoates nanoparticles as intracellular sustained drug-release vectors. J Biomater Sci 2010;21:127–40.

Cai L, Yuan MQ, Liu F, Jian J, Chen GQ. Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442. Bioresour Technol 2009;100:2265-70.

Yogesh C, Pathak B, Fulekar MH. PHA-production application and its bioremediation in environment. I Res J Environ Sci 2012;1:46-52.

Aarthi N, Ramana KV. Identification and Characterization of Polyhydroxybutyrate producing Bacillus cereus and Bacillus mycoides strains. Int J Environ Sci 2011;1:744-6.

Preethi R, Sasikala P, Aravind J. Microbial production of polyhydroxyalkanoate (PHA) utilizing fruzzzzit waste as a substrate. Res Biotechnol 2012;3:61-9.

Kumalaningsih S, Hidayat N, Aini N. Optimization of Polyhydroxyalkanoates (PHA) production from liquid bean curd waste by alcaligenes latus bacteria. J Agric Food Tech 2011;1:63-7.

Hong K, Sun S, Tian W, Chen GQ, Huang W. A rapid method for detecting bacterial polyhydroxyalkanoates in intact cells by Fourier transform infrared spectroscopy. Appl Microbiol Biotechnol 1999;51:523-6.

Oliveira FC, Dias ML, Castilho LR, Freire DMG. Characterization of poly (3-hydroxybutyrate) produced by Cupriavidus necator in solid-state fermentation. Bioresource Technol 2007;98:633-8.

Kim DY, Yun JH, Kim HW, Bae KS, Rhee YH. Purification and characterization of poly (3-hydroxybutyrate) depolymerase from a fungal isolate, Emericellopsis minima W2. J Microbiol 2002;40:129-33.

Nurbas M, Kutsal T. Production of PHB and P(HB-co-HV) Biopolymers by Using Alcaligenes Eutrophus. Iran Polymer J 2004;13:45-51.

Doi Y, Nakamura Y, Kunioka M, Soga K. 1H and 13C NMR analysis of poly (ï¢-hydroxybutyrate) isolated from Bacillus megaterium. Macromol 1986;19:2860-4.

Abd-El-Haleem AMD, AlMa’adeed MA, Al-Thani N. Physical and chemical properties of Polyhydroxyalkanotes biodegradable polymers produced in transgenic yeasts. Global J Environ Res 2007;1:69-73.

Wu L, Wang L, Wang X, Xu K. Synthesis, characterizations and biocompatibility of novel biodegradable star block copolymers based on poly[(R)-3-hydroxybutyrate] and poly(e-caprolactone). Acta Biomater 2010;6:1079–89.

Liau CP, Ahmad MB, Shameli K, Yunus WMZW, Ibrahim NA, Zainuddin N, et al. Preparation and Characterization of Polyhydroxybutyrate/Polycaprolactone Nanocomposites. Sci World J 2014.;[Article in press].

Nair AM, Annamalai K, Kannan SK, Kuppusamy S. Characterization of polyhydroxyalkanoates produced by Bacillus subtilis isolated from soil samples. Malaya J Biosci 2014;1:8–12.



How to Cite

Devi, A. B., C. V. Nachiyar, T. Kaviyarasi, and A. V. Samrot. “CHARACTERIZATION OF POLYHYDROXYBUTYRATE SYNTHESIZED BY BACILLUS CEREUS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 140-4,



Original Article(s)