GENETIC DIVERSITY ASSESSED THROUGH RAPD MARKERS IN SYZYGIUM ALTERNIFOLIUM (WT) WALP

  • T. P. Sasikala Sri Venkateswara University
  • J. Kamakshamma Dr.J.Kamakshamma Assistant Professor Department of Botany Sri Venkateswara University Tirupati

Abstract

Objective: The present study has been carried out to evaluate the genetic diversity in a representative population of Syzigium alternifolium from six different accessions of chittoor district.

Methods: Genomic DNA isolation, Polymerase Chain Reaction (PCR) amplication by using RAPD (OPJ, OPM series) primers, Agarose gel Electrophoresis, Jaccard’s similarity coefficients, and UPGMA Dendrogram.

Results: Initially, 20 decamer primers were screened, a total of 28 bands were obtained from seven reproducible primers, with a mean of four amplified bands per primer. The primers OPJ6 and OPM2 resulted 100 % polymorphism. The plant species showed an average of 70.99% polymorphism. Cluster analysis (UPGMA) was used to generate a dendrogram based on Jaccard’s similarity coefficients. A maximum similarity value of 76.5% was observed two accessions of 3 and 2 followed by 2 and 1 i.e. 71.4%. Whereas 27.8% similarity value was observed between accessions 5 and 3 and, which were found to be genetically most diverse.

Conclusion: The results suggest that, S. alternifolium possess the high level of genetic diversity, as compared to other endangered plant species. Present study concludes, RAPD is a potential molecular marker to evaluate the genetic diversity of S. alternifolium species.

 

Keywords: Syzigium alternifolium, Endemic, Endangered, Genetic diversity, RAPD markers and Polymorphism

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Author Biography

J. Kamakshamma, Dr.J.Kamakshamma Assistant Professor Department of Botany Sri Venkateswara University Tirupati
Botany department Assistant Professor

References

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How to Cite
Sasikala, T. P., and J. Kamakshamma. “GENETIC DIVERSITY ASSESSED THROUGH RAPD MARKERS IN SYZYGIUM ALTERNIFOLIUM (WT) WALP”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 4, Feb. 2015, pp. 137-40, https://innovareacademics.in/journals/index.php/ijpps/article/view/4569.
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