IN VITRO ANTIOXIDANT ACTIVITIES, TOTAL FLAVONOID, PHENOLIC AND CAROTENOID CONTENT FROM VARIOUS EXTRACTS OF FOUR SPECIES ASTERACEAE HERB
Keywords:Antioxidants, DPPH, FRAP, Asteraceae herbs, Flavonoid, Phenolic, Carotenoid
Objectives: The objectives of this research were to study antioxidant activities from various extracts of Asteraceae herbs using two methods of antioxidant assays which were DPPH (2,2-diphenyl-1-picrylhidrazyl) and FRAP (Ferric Reducing Antioxidant Power); and correlation of total flavonoid, phenolic, and carotenoid content in various extracts of Asteraceae herbs with DPPH antioxidant activities and FRAP antioxidant capacities.
Methods: Extraction was performed by reflux apparatus using different polarity solvents. The extracts were evaporated using the rotary evaporator. Antioxidant capacities were tested using DPPH and FRAP assays. Determination of total flavonoid, phenolic, and carotenoid content was performed by spectrophotometer UV-visible and their correlation with DPPH antioxidant activities and FRAP antioxidant capacities were analyzed by Pearson's method.
Results: Methanolic extract of Bidens pilosa herbs (BP3) had the highest DPPH scavenging activity with IC50 76.25 Âµg/ml, while ethyl acetate extract of B. pilosa herbs (BP2) had the highest FRAP capacity with EC50 33.50 Âµg/ml. Ethyl acetate extract of B. pilosa (BP2) had the highest total flavonoid (14.66 g QE/100 g), BP3 had the highest phenolic content (7.61 g GAE/100 g), and ethyl acetate extract of Sonchus arvensis (SA2) had the highest carotenoid content (11.92 g BE/100 g).
Conclusions: There was a positively high correlation between total phenolic with their antioxidant activity using FRAP and DPPH assays. The FRAP capacities in Artemisia vulgaris, Bidens pilosa, Ageratum conyzoides, and Sonchus arvensis herbs extracts had linear result with DPPH scavenging activities.
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