APPLE PECTIN (AP) INDUCED APOPTOSIS VIA NITRIC OXIDE (NO) IN HUMAN PROSTATE CANCER CELLS DU145
Objective: Apoptosis or programmed cell death is a physiological process in which cells die. Many cancer chemical drugs induce apoptosis to omit cancer cells. Since prostate cancer is one of the most common cancers among men, it is important to develop some natural ways to stop it. Plant derivatives are capable of inducing apoptosis in cancer cells. Pectin, a carbohydrate-rich compound of plant cells, is one of these derivatives which show apoptotic effects on cancer cells. Here, we studied the effect of apple pectin (AP) to induce apoptosis in human prostate cancer cells.
Methods: The cellular viability was investigated with MTT, cell cycle analysis and AO/EB double staining. The amount of NO release was determined and apoptosis was studied through western blotting of the proteins which takes part in the cell death pathway.
Results: The results indicated that AP strongly suppressed Du145 cells proliferation. It also caused significant increase of NO release compared with control group. Treatment by different concentrations of AP led to the enhancement of active caspase-3 levels and Bax/Bcl2 Ratio.
Conclusion: These finding suggest that AP has the potential to induce apoptosis in prostate cancer cells (DU145) through increasing the release of NO which may be related to the mitochondrial apoptosis pathway.
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