SPECTROSCOPIC ANALYSIS AND ANTIBACTERIAL EFFICACY OF BIOACTIVE COMPOUNDS FROM LIMONIA ACIDISSIMA L. FRUIT EXTRACT AGAINST CLINICAL PATHOGENS

Authors

  • Srinivasan Ponnuraj RESEARCH SCHOLAR, DEPT. OF BIOTECHNOLOGY, KARPAGAM UNIVERSITY, POLLACHI MAIN ROAD, COIMBATORE-21, TAMIL NADU, INDIA
  • Dineshbabu Jaganathan RESEARCH SCHOLAR, DEPT. OF BIOTECHNOLOGY, KARPAGAM UNIVERSITY, POLLACHI MAIN ROAD, COIMBATORE-21, TAMIL NADU, INDIA
  • Manimekalai Kanagarajan RESEARCH SCHOLAR, DEPT. OF BIOTECHNOLOGY, KARPAGAM UNIVERSITY, POLLACHI MAIN ROAD, COIMBATORE-21, TAMIL NADU, INDIA
  • Teepica Priya Darsini Deivamarudachalam ASSOCIATE PROFESSOR, DEPT. OF BIOTECHNOLOGY, KARPAGAM UNIVERSITY, POLLACHI MAIN ROAD, COIMBATORE-21, TAMIL NADU, INDIA

Keywords:

Limonia acidissima, Phytochemical, Bioactive compounds, FT-IR, GC-MS, Antibacterial activity

Abstract

Objective: To determine the phytochemical composition using spectral analysis and antibacterial activity of Limonia acidissima fruit methanol extract.

Methods: Phytochemical analysis was performed to determine the total alkaloids, saponins, total phenols and flavonoids content of fruit extract. Secondary metabolite compounds were determined performed by FT-IR and GC-MS analysis. Antibacterial activity assay was performed using agar well diffusion technique.

Results: The alkaloid and saponins contents of the crude methanol extract were found to be 38.47 g/100 g and 0.13 g/100 g dry matter respectively. The LF methanol extract showed total phenol contents of 33.38 µg/mg and the flavonoids was found to be 33.80 µg/mg extract. FT-IR analysis indicated the presence of phenols, alkanes, amino acids, α, β-unsaturated esters, alkenes, nitro compounds, aromatics, aliphatic amines, carboxylic acid, alkenes, and alkyl halides functional groups. The GC-MS analysis revealed the presence of linoleic acid, octadecanoic acid, hexadecanoic acid, maltol, vinly guaiacol, furanone, and ascorbic acid. The antibacterial activity assay showed dose dependent inhibition against clinical pathogens.

Conclusion: This study elucidated the prospective value of L. acidissima fruit as a nutritional and medicinal source.


 

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Published

01-03-2015

How to Cite

Ponnuraj, S., D. Jaganathan, M. Kanagarajan, and T. P. D. Deivamarudachalam. “SPECTROSCOPIC ANALYSIS AND ANTIBACTERIAL EFFICACY OF BIOACTIVE COMPOUNDS FROM LIMONIA ACIDISSIMA L. FRUIT EXTRACT AGAINST CLINICAL PATHOGENS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 383-9, https://innovareacademics.in/journals/index.php/ijpps/article/view/4711.

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