IN VITRO ANTIOXIDANT ACTIVITY OF <em>PROSOPIS CINERARIA</em> LEAVES AGAINST H<sub>2</sub>O<sub>2</sub> INDUCED OXIDATIVE STRESS IN GOAT LIVER SLICES
Keywords:
in vitro models, Prosopis cineraria, Liver slices, Antioxidant activity, Hydrogen peroxide, Oxidative stress, Vanni, Enzymic antioxidants, Non-enzymic antioxidantsAbstract
Objective: To evaluate the antioxidant status of goat liver slices treated with standard oxidant H2O2in the presence and the absence of methanolic leaf extract of Prosopis cineraria.
Methods: Precision-cut goat liver slices were challenged with oxidant H2O2 with or without the methanolic leaf extract of Prosopis cineraria. The activity of enzymic antioxidants and the levels of non-enzymic antioxidants were analysed in the homogenate of liver slices.
Results: The levels of antioxidants were drastically decreased in oxidant treated group compared to untreated control, whereas there was a significant increase in the antioxidants levels in the methanolic leaf extract treated group.
Conclusion: The organ slices represent the multicellular, structural and functional features of in vivo tissues and therefore represent as a good in vitro model. The present study revealed that leaf extract improved the antioxidant status of the goat liver slices and also protected against oxidative damage.
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