CHARACTERIZATION AND ANTIBACTERIAL STUDIES OF LEAF ASSISTED SILVER NANOPARTICLES FROM CARICA PAPAYA: A GREEN SYNTHETIC APPROACH
Keywords:Green synthesis, Silver nanoparticles, Carica papaya, Characterization and antibacterial activity
Objective: The aim of this study is to synthesis silver nanoparticles using Carica papaya leaf extracts and testing its antibacterial activity.
Methods: Silver nanoparticles (Agnps) were prepared by treating Carica papaya leaf extracts with 1 mm silver nitrate (AgNO3). The bioreduced silver nanoparticles were characterized by ultraviolet visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR) and the size and shape of the silver nanoparticles were obtained from transmission electron microscopy (TEM). The synthesized silver nanoparticles were tested for their antibacterial activity by disc diffusion method.
Results: From the UV spectrum, it was observed that the peak at 430 nm showing the reduction of silver ions to stable silver nanoparticles. The size of the biosynthesized nanoparticles was in the range of 10-50 nm obtained from TEM image. FTIR measurements provided the bands at different wave numbers, indicating the stretching of alcohols, carboxylic acids, esters and ethers. The synthesized silver nanoparticles exhibited an excellent bactericidal activity against bacterial strains like Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.
Conclusion: Green synthesis of silver nanoparticles using papaya leaf extract exhibited effective antibacterial property.
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