ANTIDIABETIC ACTIVITY ON METHANOLIC EXTRACTS OF SCHREBERA SWIETENOIDES ROXB. ROOTS IN STREPTOZOTOCIN INDUCED DIABETIC RATS
Objective: The aim of study was to evaluate acute toxicity and antidiabetic activity of methanolic extract of Schrebera swietenoides roots in streptozotocin induced diabetic rats.
Methods: Acute toxicity of the methanolic extracts of Schrebera swietenoides roots was studied in Wistar albino rats at a dose level upto 2 g/kg b.w. The animals were also closely examined for signs of intoxication, lethargy, behavioral modification and morbidity. The plant was evaluated for antidiabetic activity against Streptozotocininduced diabetic model in different concentrations of 100,200 and 400 mg/kg body weight. The mean and percent blood glucose reduction levels were calculated and comparable with the standard drug, glibenclamide (0.45 mg/kg b. w).
Results: The extracts did not produce any toxic signs during the observation period for 24 h. The oral administration of 400 mg/kg b. w of methanolic extract of Schrebera swietenoides roots showed significant (P<0.05) decrease in blood glucose levels up to 18th h, more significant (P<0.01) decrease in blood glucose levels at 4th and 12th h and highly significant (P<0.001) decrease in blood glucose levels at 8th h respectively.
Conclusions: From the studies it is concluded that, the methanolic extract of Schrebera swietenoides roots is suitable to prepare ayurvedic formulations for the treatment of diabetes.
2. Prakasam A, Sethupathy S, Pugalendi KV. Hypolipidaemic effect of Casearia esculenta root extracts in streptozotocin-induced diabetic rats. Pharmazie 2003;58:828-32.
3. Balu S, Alagesboopathy C, Madhava S. Ethnobotany and medicinal plant of india subcontinent, Scientific publishers, Jodhpur; 2003. p. 359.
4. Khare CP. Indian Medicinal Plants-An illustrated dictionary. spinger publications; 2007. p. 589.
5. Madhav seetty K. Flowering Plants of chittoor District, Andhra Pradhesh India; 2008. p. 194.
6. Lakshman Kumar D, Rao KNV, Bindu Madhavi, Satish Kumar D, David B. Alpha glucosidase inhibitory activity of Wrightia tinctoria Roxb and Schrebera swietenoides Roxb bark extract. Res J Pharmacol Pharm 2011;3:22-6.
7. Lakshman Kumar D, Rao KNV, Bindu Madhavi, Satish Kumar D, David B. Antioxidant activity of Wrightia tinctoria Roxb and Schrebera swietenoides Roxb bark extract. J Pharm Res 2011;4:396-7.
8. Ghosh MN. Fundamentals of Experimental Pharmacology. 2nd Edn. Scientific Book Agency, Calcutta; 1984. p. 178-210.
9. Gonzalez M, Zarzuelo A, Gamez MJ, Utrilla MP, Jimenez J, Osuna I. Hypoglycemic activity of olive leaf. Planta Med 1992;58:513-5.
10. Padmini-kedar, Chakrabharathi CH. Effect of bitter gourd (Momordica charantia) seed and glibenclamide in streptozotocin induced diabetes mellitus. Indian J Exp Biol 1982;20:181-4.
11. Regi Raphael K, Sabu MC, Ramadasan Kuttan. Hypoglycemic effect of methanol extract of phyllanthus amarus schum and Thonn on alloxan induced diabetes mellitus in rats and its relation with antioxidant potential. Indian J Exp Biol 2002;40:905-9.
12. Khosla P, Gupta DD, Nagpal RK. Effect of Trigonella foenum graecum (fenugreek) on blood glucose in normal and diabetic rats. Indian J Physiol Pharmacol 1995;39:173â€“4.
13. Philip D Mayne. Carbohydrate metabolism in clinical chemistry in diagnosis and Treatment, ELBS; 1994.
14. Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem 1969;6:24-5.
15. Raabo E, Terkildsen TC. On the enzymatic determination of blood glucose. Scand J Clin Lab Invest 1960;12:402-7.
16. Snedecor GW, Cochran WG. The comparison of two samples, in Statistical Methods, Iowa State University Press: Ames, Iowa; 1967. p. 91â€“119.
17. Woodson RF. Statistical methods for the analysis of biomedical data series in probability and Mathematical statistics, wiley, New York; 1987. p. 315.
18. Zar JH. Biostatistical Analysis, Prentice-Hall. Inc., Englewood Chiffs. NJ; 1974. p. 151.
19. Manjusha, Aggarwal N, Nitesh, Gupta P. Effect of petroleum ether extract of Sesbania sesban (Merr.) roots in streptozotocin (STZ) induced diabetes in mice. Asian Pac J Trop Biomed 2012;673â€“8.
20. Patel DK, Prasad SK, Kumar R, Hemlatha S. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed 2012;2:320â€“30.
21. Wollenweber LE, Cody V, Middletone EJ, Harborne JB, Bertz A. Plant flavonoid in biological and medicinell: biochemical, cellular and medicinal properties. Prog Clin Biol Res 1998;280:1â€“461.