ANTIULCEROGENIC AND ANTIULCER ACTIVITIES OF DISSOTIS THOLLONII (MELASTOMATACEAE) LEAVES IN RATS

  • Ateufack Gilbert Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang P. O. box 67 Dschang Cameroon
  • Mikwangock Domegne Hauberline M. Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang P. O. box 67 Dschang Cameroon
  • Dongmo Feudjio Breuil R Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang P. O. box 67 Dschang Cameroon
  • Yousseu Nana William Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang P. O. box 67 Dschang Cameroon
  • Kamanyi Albert Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang P. O. box 67 Dschang Cameroon

Abstract

Objective: Dissotis thollonii is used in Cameroonian ethnomedicine to cure diseases such as inflammations, pregnancy control, diarrhea and gastric ulcer. The aqueous and methanol leaf extracts were evaluated for their anti ulcerogenic and antiulcer activities.

Methods: The extracts were administered at the doses of 125, 250 and 500 mg/kg to evaluate their effects on gastric ulcer induced by the HCl/ethanol mixture, indomethacin and acetic acid in rats. Ranitidine, Maalox and Misoprostol were used as standard drugs. Histopathological examination and nitric oxide level was performed to evaluate the basic action mechanism of Dissotis thollonii.

Results: Oral administration of the plant dose-dependently prevented HCl/ethanol-induced gastric ulcers (72.15 to 100 % for aqueous extract and 68.78 to 89.60 % for methanol extract), Indomethacin (51.13 to 100 % for aqueous extract and-32.33 to 58.45 % for methanol extract). The extracts also promoted the healing and cicatrization process in chronic gastric ulcer induced by acetic acid and increased the NO level in plasma. Histological studies of the gastric wall revealed that ulcer control group exhibited severe damage of the gastric mucosa, compared to rats pre-treated with extracts, which comparatively showed gastric mucosal protection.

Conclusion: Dissotis thollonii possess protective and healing activities in rats. The anti ulcerogenic activity may be attributed to the stimulation of the prostaglandins synthesis and antiulcer property to increase NO level in plasma. Histological investigation of gastric lesion shows that the plant stimulates the cicatrizing process. These results supported the ethnomedicinal uses of Dissotis thollonii in the treatment of gastric ulcers.

 

Keywords: Dissotis thollonii, Anti ulcerogenic, Antiulcer

Downloads

Download data is not yet available.

References

1. Venkat R, Kola V, Sowmya U, Jayapal G, Anirudh K. Evalution of anti-ulcer activity of Momordica charactia in rats. Int J Pharm Biol Sci 2011;1:1-16.
2. Uyanikoglu A, Danalioglu A. The ethiological factors of duodenal and gastric ulcer. Pecptic Ulcer Disease 2011;6:93-7.
3. konturek SJ, Konturek PC, Buzozowski T. Prostaglandins and ulcer heating. J physiol pharmacol 2005;55(5):3-31.
4. Mota KSDL, Pita JCLR, Estevam EC. Evaluation of the toxicity and antiulcerogenic activity of the ethanol extract of Maytenus ostusifolia Mart. leaves. Brazilian J pharmacognosy 2008;18(3):441-6.
5. Lanas A. Non steroidal antiinflammatory drugs and cyclooxygenase inhibition in the gastrointestinal tract: a trip from peptic ulcer to colon cancer. Am J Med Sci 2009;338(2):96-106.
6. Rao CV, Ojha SK, Radhakrishna K. Antiulcer activity of Ulteria ralicifolia rhizome extract. J Ethnopharmacol 2009;1(3):243-9.
7. Luiz-Ferreira A, De Almeida ACA, Cola M. Mechanisms of the gastric antiulcerogenic activity of Anarcadium humile on ethanol-induced acute gastric mucosal injury in rats. Molecules 2010;15(10):7153-66.
8. Abdulla MA, Ahmed KAA, Al-Bayaty FH, Massod Y. “Gastroprotective effect of Phyllanthus niruri leaf extract against ethanol-induced gastric mucosal injury in rats”. Afr J Pharm Pharmacol 2010;4(5):226-30.
9. Wasman SQ, Mahmood AA, Salehhuddin H, Zahra AA, Salmah I. “Cytoprotective activities of Polygonum minus aqueous leaf extract on ethanol-induced gastric ulcer in rats”. J Med Plant Res 2010;4(24):2658-65.
10. Lais GP, Fermanda MR, Valdercir FX, Anne LD. Antioxidant activity and total phenols from the methanolic extract of Miconia albicans (sw.) Triana leaves. Molecules 2011;16:9439-50.
11. Susanti D, Sirat HM, Ahmad F, Ali RM, Aimi N, Kitajima M. Antioxidant and cytotoxic flavonoids from the flowers of Melastoma malabathricum L. Food Chem 2007;103:710-6
12. Cheng JT, Hsu FL, Chen HF. Antihypertensive principles from the leaves of Malastoma candidium. Planta Med 1993;59:405-7.
13. Almalraj T, Ignacimuthu S. Evaluation of the hypoglycaemic effect of Memecylon umbellatum in normal and alloxan diabetic mice. J Ethnopharmacol 1998;62:247-50.
14. Nicholl DS, Daniels HM, Thabrew MI, Grayer RJ, Simmonds MSJ, Hughes RD. In vitro studies on the immunomodulatory effect extracts of Osbeckia aspera. J Ethnopharmacol 2001;78:39-44.
15. Abere TA, Okoto PE, Agoyero FO. Antidiarrhoea and toxicological evaluation of the leaf extract of Dissotis rotundifolia triana (Melastomataceae). BMC Complementary Altern Med 2010;10:71-7.
16. Yoshida T, Arioka H, Fujita T, Chen XM, Okuda T. Monomeric and dimeric hydrolysable tannins from two Melastomataceous species. Phytochemistry 1994;37:863-6.
17. Isaza JH, Ito H, Yoshida T. A flavonol glycoside-ligan ester and accompanying acylated glucosides from Monochaetum muliflorum. Phytochemistry 2001;58:321-7.
18. Calderon AI, Terreaux C, Schenk K, Pattison P, Burdette JE, Pezzuto JM, et al. Isolation and structure elucidation of an isoflavone and a sesterterpen acid from Henriettella fascicularis. J Nat Prod 2002;65:1749-53.
19. Ndjateu FST, Tsafack RBN, Awouafack MD, Wabo HK, Tene M, Tane P, et al. Antimicrobial and antioxidant activities of extracts and ten compounds from three Cameroonian medicinal plants. Dissotis perkinsiae (Malatomataceae), Adenocarpus manii (Fabaceae) and Barteria fistulosa (Passifloraceae). S Afr J Bot 2014;91:37-42.
20. Nono RN, Barboni L, Teponno RB, Quassinti L, Bramucci M, Vitali LA, et al. Antimicrobial, antioxidant, anti-inflammatory activities and phytoconstituents of extracts from the roots of Dissotis thollonii Cogh. (Melastomataceae). S Afr J Bot 2014;93:19-26.
21. Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 2001;5(1):62-71.
22. Ateufack G, Nguelefack TB, Wabo HK, Tane P, Kamanyi A. Antiulcerogenic activity of 1-hydroxy-3,7,8-thrimuthoxy xanthone isolated from the methanol extract of Anthocleista vogelii Planch. In rat. Ulcer 2014;1-6.
23. Devaraj VC, Gopala, Krishna B, Viswanatha GL, Satya P, Vinay-Babu SN. Protective effect of leaves of Raphinus sativus Linn. On experimentally induced gastric ulcers in rats. Saudi Pharm J 2011;19:171-6.
24. Takagi K, okabe S, Saziki R. A new method for the production of chronic gastric ulcer in rats and the effect of several drugs on its healing. Jpn J Pharmacol 1969;19:418-26.
25. Demir S, Yilmaz M, Köseoglu M, Akalin N, Aslan D, Aydin A. Role of free radicals in peptic ulcer and gastritis. Turkish J Gastroenterol 1988;23(2-3):299-304.
26. Yamahara J, Mochizuki M, Rong HQ, Matsuda H, Fujimura H. The antiulcer effect in rat of ginger constituents. J Ethnopharmacol 1988;3(2):209-16.
27. Marhuenda E, Martin MJ, Alarcon De La Lastra C. Antiulcerogenic activity of Aescine in different experimental models. Phytother Res 1993;23(1):13-6.
28. Franke A, Teyssen S, Singer MV. Alcohol-related diseases of the esophagus and stomach. Dig Dis 2005;23(3-4):204-13.
29. Oluwole FS, Ayo JA, Omolaso BO, Emikpe BO, Adesanwo JK. Methanol extract of Tetracera potatoria, an antiulcer agent increases gastric mucus secretion and endogenous antioxydants. Niger J Physiol Sci 2008;23(1-2):79-83.
30. Al-Attar AM. Protective effect of Avicennia alba leaves extract on gastric mucosal damage induced by ethanol. Res J Med Plant 2011;5(4):477-90.
31. Flemstrom G, Garner A, Nylander O, Hurstt BC, Heyling JR. Surface epithelial HCO3 transport by mammalian duodenum in vivo. Am J Physiol Gastrointestinal Liver Physiol 1982;243:G348-58.
Statistics
465 Views | 630 Downloads
How to Cite
Gilbert, A., M. D. Hauberline M., D. F. Breuil R, Y. N. William, and K. Albert. “ANTIULCEROGENIC AND ANTIULCER ACTIVITIES OF DISSOTIS THOLLONII (MELASTOMATACEAE) LEAVES IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 8, June 2015, pp. 62-69, https://innovareacademics.in/journals/index.php/ijpps/article/view/5665.
Section
Original Article(s)