SIMULTANEOUS ESTIMATION OF CAFFEIC AND CHLOROGENIC ACID CONTENT IN AMMI MAJUS SEED BY TLC AND HPLC

Authors

  • Aveen Nozad Adham Hawler Medical University/ Collage of Pharmacy

Keywords:

Ammi majus, TLC, HPLC, Caffeic acid, Chlorogenic acid

Abstract

Objective: The present investigation formulated in order to identify and quantify of caffeic and chlorogenic acid content in Ammi majus seed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC).

Methods: In the present study identification and quantification of caffeic and chlorogenic acid in Ammi majus seeds were investigated. Identification of these phenolic acids were done by using thin layer chromatography performed on a sheet of aluminium plates of silica gel as a stationary phase, suitable solvent systems which is Toluene: Ethyl acetate: Formic acid: Water (15:90:5:5), also use UV light and 5 % Fecl3 for detection. This identification was further augmented by using high performance liquid chromatography and then quantified by using C18 column, isocratic mobile phase (acetonitrile: water (0.5% orthophosphoric acid) 15:85) and UV/Visible 320 nm detector.

Results: Thin layer chromatography analysis of seed extracts revealed the presence of caffeic and chlorogenic acid in Ammi majus seed. The HPLC method was validated for linearity, accuracy, precision and limit of detection and quantification. The percentage of caffeic acid 0.031% in higher quantity than chlorogenic acid. Calibration curve was linear over concentration 5-200 µg/ml with correlation coefficients 0.998 and 0.999 for caffeic and chlorogenic acid. The relative standard deviation of the method was less than 2% for intra and inter-day precision assay and the average recoveries were between 97.47-101.92%. The proposed method was found to be linear, accurate, and precise.

Conclusion: This study confirms the presence of caffeic and chlorogenic acid in Ammi majus seed extracts and the percentage of caffeic acid higher than chlorogenic acid.

 

Downloads

Download data is not yet available.

Author Biography

Aveen Nozad Adham, Hawler Medical University/ Collage of Pharmacy

Department of pharmacognosy

References

Samani AM, Farkhad KN, Azimi N, Fasihi A, Ahandani AE, Kopaei RM. Medicinal plants with hepatoprotective activity in Iranian folk medicine. Asian Pac J Trop Biomed 2015;5(2):146-57.

Shahbaz ES. Medicinal plants of Kurdistan Iraq. 1st ed. Kurdistan: Dohuk Governorate printing press; 2013. p. 44-5.

Akhtar P, Ali M, Sharma MP, Waris M, Hasan H, Ali B, et al. Development of quality standards of Ammi majus L. Fruit J Exp Sci 2010;1(11):20-4.

Selim YA, Ouf NH. Antiâ€inflammatory new coumarin from the Ammi majus L. Org Med Chem Lett 2012;2:1â€4.

Ekiert H, Gomolka E. Coumarin compounds in Ammi majus callus cultures. Pharmazie 2000;55:684-7.

Abdul-Jalil ZT, Saour K, Nasser AA. Phytochemical study of some flavonoids present in the fruits of two Ammi L. species wildly grown in Iraq. Iraqi J Pharm Sci 2010;19(1):48-57.

Al-Hadidi AK, Al-Numan YA, Al-Daody CA. Interaction between some phenolic compounds in ammi majus herb: (khillah) extracts and antibiotics against some selected bacterial isolates in vitro. Raf J Sci 2013;24(2):17-30.

Fakim GA. Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Aspects Med 2006;7(1):1-93.

Alupuli A, Calinescu I, Lavric V. Ultrasonic vs. microwave extraction intensification of active principles from medicinal plants. AIDIC Conference Series 2009;09:1-8.

ICH. Guideline Q2A, text on validation of analytical procedures: federal register, rockville, MD, USA; 1995;60(40):11260-2.

ICH. Guideline Q2B, validation of analytical procedures: methodology, federal register, rockville, MD, USA; 1997;62(96):27463–7.

Aljadi AM, Yusoff KM. Isolation and identification of phenolic acids in Malaysian honey with antibacterial properties. Turk J Med Sci 2003;33:229-36.

Popova M, Bankova V, Butovska D, Petkov V, Damyanova NB, Sabatini AG. Validated methods for the quantification of biologically active constituents of poplartype propolis. Phytochem Anal 2004;15:235-40.

Volpi N. Separation of flavonoids and phenolic acids from propolis by capillary zone electrophoresis. Electrophoresis 2004;25:1872-8.

Calixto JB. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents): The medicinal use of herbal drugs. Braz J Med Biol Res 2000;33:179-89.

Published

01-06-2015

How to Cite

Adham, A. N. “SIMULTANEOUS ESTIMATION OF CAFFEIC AND CHLOROGENIC ACID CONTENT IN AMMI MAJUS SEED BY TLC AND HPLC”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 6, June 2015, pp. 263-7, https://innovareacademics.in/journals/index.php/ijpps/article/view/5912.

Issue

Section

Original Article(s)