SIMPLE HPLC-UV METHOD FOR DETERMINATION OF METFORMIN IN HUMAN PLASMA AND ERYTHROCYTES APPLICATION TO THERAPEUTIC DRUG MONITORING

  • Mohammed Abdessadek Department of Endocrinology, Diabetology and Metabolic Diseases, Hassan II University Hospital of Fez, Morocco
  • Az. Eddine Tadmori Hassan II University Hospital of Fez
  • Ahmed El. Attari Hassan II University Hospital of Fez
  • Martin Diarra Hassan II University Hospital of Fez
  • Rabia Magoul Sidi Mohammed Ben Abdellah University
  • Farida Ajdi Hassan II University Hospital of Fez
  • Seloua El. Ouezzani Sidi Mohammed Ben Abdellah University
  • Youssef Khabbal Hassan II University Hospital of Fez

Abstract

Objective: The aim of this study was to develop a simple, rapid, efficient, cost effective and reproducible, stability indicating reverse phase high performance liquid chromatography method (RP-HPLC) for dosage of metformin in human plasma and erythrocytes.

Methods: In this method, the plasma or erythrocyte proteins were precipitated using Perchloric acid: acetonitrile (50 % v/v) mixture and the supernatant liquid were injected into the HPLC system. The separation was achieved with a symmetry C8 column with the mobile phase containing 10 % water and 90 % sodium dihydrogen phosphate buffer (5.8 mM), the pH was adjusted to 3.8 with Phosphoric acid. The temperature was elevated to 25 °C. The detection was done by a UV detector at 232 nm.

Results: The retention time was observed at around 4.412 min for metformin and 6.022 for lansoprazole an internal standard (IS). The response was linear over a range of 2-32µg ml-1, the coefficient of determination (r²) was found to be (r² =0. 9988). The lowest limit of quantification and detection was 0.1 µg/ml and 0.3µg/ml respectively. No endogenous substances were found to interfere with the peaks of the drug. The intra-day and inter-day coefficient of variations was 2.1 % or less for all the selected concentrations. The relative errors at all the studied concentrations were 3.5 % or less.

Conclusion: The HPLC method described in this article was simple, selective, reproducible, linear, and precise, it can be applied for therapeutic drug monitoring of metformin in human plasma and erythrocytes.

 

Keywords: Metformin, Method, Plasma, Erythrocytes, HPLC, C8 column

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Abdessadek, M., A. E. Tadmori, A. E. Attari, M. Diarra, R. Magoul, F. Ajdi, S. E. Ouezzani, and Y. Khabbal. “SIMPLE HPLC-UV METHOD FOR DETERMINATION OF METFORMIN IN HUMAN PLASMA AND ERYTHROCYTES APPLICATION TO THERAPEUTIC DRUG MONITORING”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 11, Sept. 2015, pp. 35-39, https://innovareacademics.in/journals/index.php/ijpps/article/view/6086.
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