UNRAVELING THE POTENTIAL PHYTOCHEMICAL COMPOUNDS OF GYMNEMA SYLVESTRE THROUGH GC-MS STUDY
Objective: To profile the chemical composition of ethanolic extract of Gymnema sylvestre through Gas Chromatography-Mass Spectrometry technique.
Methods: The chemical compositions of the plant leaf extracts of G. sylvestre were investigated using Gas Chromatographyâ€“Mass Spectroscopy (Scion 436-GC Bruker model coupled with a Triple, quadruple mass spectrophotometer) and NIST-MS library.
Results: GC-MS analysis of leaf extracts revealed the existence of Terpenes, alcohols, fatty acids, amine and sterols. The highest % Peak area is hexadecanoic acid, Î±-Santoline alcohol, recorded the next highest % peak area of 9.05. Major of the compounds belongs to terpeneoid group, namely 6-Octen-1-ol, 3,7-dimethyl, Isophytol, Squalene, Nerolidol, Î²-Amyrin and Cedrene-V6 which constitutes 30.7% of the peak area. The presence of Î±-Tocopherol-Î²-D-mannoside and Vitamin E also identified through this study.
Conclusion: From the above finding we can interpret that the G. sylvestre contained a considerable amount of phytoconstituents especially terpenoids. In future, this study will be helpful for the quantitative analysis of phytochemicals as well as formulation studies.
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