ANTIOXIDANT AND CELL ADHESION PROPERTIES OF COLLAGEN FROM JELLYFISH ACROMITUS FLAGELLATUS

  • Maduraiveeran Hemavathi University of Madras
  • Palani Dhayananthan University of Madras
  • Sivasamy Sivaranjani University of Madras
  • Samson Kumar University of Madras
  • Shiva Shakthi University of Madras
  • Chinnsamy Arulvasu Department of Zoology, University of Madras, Guindy Campus, Chennai 600025, Tamilnadu, India

Abstract

Objective: Collagen is an important biomaterial in medical applications due to its special characteristics of biodegradability and weak antigenicity. Collagens from many sources possess specific biological properties that make potential ingredient of health promoting food. Jellyfish is a rich source of collagen, which has high nutritious and medicinal value. In this study, the cell adhesion and antioxidant activity of the collagen from jellyfish Acromitus flagellatus were evaluated for the development of novel pharmaceutical agents.

Methods: The pepsin solubilized (ps) collagen of the jellyfish A. flagellatus were subjected to protein quantification and profiling. The collagen was evaluated for its cell adhesion and anti oxidant free radical scavenging potential by in vitro assay.

Results: The protein content of the jellyfish collagen was estimated to be 0.013 mg/ml. The protein profile of crude collagen protein revealed the molecular weight to be in the range of 205 kDa to 29 kDa. The collagen showed cells adherent property in a dose dependent manner with increase in the coating concentrations at 20-100 μg/ml. It also showed a potent free radical scavenging activity in the dose dependent with a highest activity at the concentration of 294.63 μg/ml.

Conclusion: Our results suggest that the collagen isolated from A. flagellatus can be used potentially as an alternative source of collagen used in various biomedical applications which is more reliable and easily available.

 

Keywords: Jelly fish, Collagen, Antioxidants, Cell adhesion

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Author Biography

Maduraiveeran Hemavathi, University of Madras
department of zoology

References

1. Alberts B, Johnson A, Lewis J, Raff M, Roberts K. Molecula Biology of the Cell. Garland Science: New York; 2002.
2. Wood A, Ogawa M, Portier M, Schexnayder M, Shirley M, Losso JN. Biochemical properties of alligator (Alligator mississippiensis) bone collagen. Comp Biochem Physiol Part B: Biochem Mol Biol 2008;151:246-9.
3. Birk DE, Fitch JM, Babiarz JP, Linsenmayer TF. Collagen type I and type V are present in the same fibril in the avian corneal stroma. J Cell Biol 1988;6:999-1008.
4. Kobayashi K, Maehata Y, Kawamura Y, Kusubata M, Hattori S, Tanaka K. Direct assessments of the antioxidant effects of the novel peptide on reactive oxygen species using electron spin resonance spectroscopy. J Pharm Sci 2011;116:97-06.
5. Krishna OD, Jha AK, Jia X, Kiick KL. Integrin-mediated adhesion and proliferation of human MSCs elicited by a hydroxyproline-lacking, collagen-like peptide. Biomaterials 2011;32:6412-24.
6. Ramshaw JA, Peng Y, Glattauer V, Werkmeister JA. Collagens as biomaterials. J Mater Sci Mater Med 2009;20:3-8.
7. Hiraoka Y, Kimura Y, Ueda H, Tabata Y. Fabrication and biocompatibility of collagen sponge reinforced with poly (glycolic acid) fiber. Tissue Eng 2003;9:1101-12.
8. Lee CH, Singla A, Lee Y. Biomedical applications of collagen. Int J Pharm 2001;221:1-22.
9. Chvapil M, Kronenthal RL, Van Winkle W. Medical and surgical applications of collagen. Int Rev Connect Tissue Res 1973;6:1-61.
10. Neklyudov AD. Nutritive fibers of animal origin: Collagen and its fractions as essential components of new and useful food products. Appl Biochem Microbiol 2003;39:229-38.
11. Zhuang Y, Hou H, Zhao X, Zhang Z, Li B. Effects of collagen and collagen hydrolysate from jellyfish (Rhopilema esculentum) on mice skin photoaging induced by UV irradiation. J Food Sci 2009;74:138-83.
12. Gomez Guillen MC, Lopezz Caballero ME, Montero MP. Functional and bioactive properties of collagen and gelatin from alternative sources: a review. Food Hydrocolloids 2011;25:1813-27.
13. Cuneo F, Costa Paiva L, Pinto Neto AM, Morais SS, Amaya Farfan J. Effect of dietary supplementation with collagen hydrolysates on bone metabolism of postmenopausal women with low mineral density. Maturitas 2010;65:253-7.
14. Deyl Z, Miksík I. Comparison of different electrokinetic separation modes applicable to a model peptide mixture (collagen type I and III CNBr fragments). J Chromatogr B: Biomed Sci Appl 2000;745:251-60.
15. Miura S, Kimura S. Jellyfish mesogloea collagen characterization of molecules a α1α2α3 heterotetramers. J Biol Chem 2005;260:15352-6.
16. Kimura S, Ohno Y, Miyauchi Y, Uchida. Fish skin type I collagen: wide distribution of an α3 subunit in teleosts. Comp Biochem Physiol 1987;88B:27-34.
17. Nagai T, Suzuki N, Nagashima T. Collagen from common minke whale (Balaenoptera acutorostrata) unesu. Food Chem 2008;111:296-301.
18. Zhang Y, Liu W, Li G, Shi B, Miao Y, Wu X. Isolation and partial characterization of pepsin-soluble collagen from the skin of grass carp (Ctenopharyngodon idella). Food Chem 2007;103:906-12.
19. Gumbiner BM. "Cell adhesion: the molecular basis of tissue architecture and morphogenesis". Cell 1996;84:345-57.
20. Cohen MB, Grieblin TL, Ahaghotu CA, Rokhlin OW, Ross JS. Cellular adhesion molecules in urologic malignancies. Am J Clin Pathol 1997;107:56.
21. Nagai T, Ogawa T, Nakamura T, Ito T, Nakagawa H, Fujiki K, et al. Collagen of edible jellyfish exumbrella. J Sci Food Agric 1999;79:855-8.
22. Reddy GK, Enwemeka CS. A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem 1996;29:225-9.
23. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976;72:248-54.
24. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4”. Nature 1970;227:680-5.
25. Elefteriou F, Exposito JY, Garrone R, Lethias C. Cell adhesion to tenascin-X mapping of cell adhesion sites and identification of integrin receptors. Eur J Biochem 1999;263:840-8.
26. Binsan W, Benjakul S, Visessanguan W, Roytrakul S, Tanaka M, Kishimura H. Antioxidative activity of mungoong, an extract paste, from the cephalothorax of white shrimp (litopenaeus vannamei). Food Chem 2008;106:185-93.
27. Krishnan S, Perumal P. Preparation and biomedical characterization of jellyfish (Chrysaora Quinquecirrha) collagen from southeast coast of india. Int J Pharm Pharm Sci 2013;5:698-701.
28. Saito E, Fujimoto M, Hasegawa M, Komura K, Hamaguchi Y, Kaburagi Y, et al. CD19-dependent B lymphocyte signaling thresholds influence skin fibrosis and autoimmunity in the tight-skin mouse. J Clin Invest 2002;109:1453-62.
29. Cui F, Xue C, Li Z, Zhang Y, Dong P, Fu X, et al. Characterization and subunit composition of collagen from the body wall of sea cucumber Stichopus japonicus. Food Chem 2007;100:1120-5.
30. Goldner R, Burnett JW, Stone J, Dilaimy MS. The chemical composition of sea nettle nematocysts. Proc Soc Exp Biol Med 1969;131:1386-8.
31. Anderluh G, Macek P. Cytolytic peptide and protein toxins from sea anemones (Anthozoa: Actiniaria). Toxicon 2002;40:111-24.
32. Addad S, Exposito JY, Faye C, Ricard Blum S, Lethias C. Isolation, characterization and biological evaluation of jellyfish collagen for use in biomedical applications. Mar Drugs 2011;9:967-83.
33. Barzansky B, Lenh M. On the chemical composition and developmental role of the mesoglea of Hydra. Am Zool 1974;14:575-81.
34. Schmid V, Bally A. Species specificity in cell substrate interactions in medusae. Dev Biol 1988;129:537-81.
35. Haller M. Isolation and partial purification of hydrozoan collagen and its relevance for species-specific cell adhesion. Diploma Thesis, University of Basel; 1989.
36. Schmid V, Bally A, Weber C. The extracellular matrix (mesoglea) of hydrozoan jellyfish and its ability to support cell adhesion and spreading. Hydrobiologia 1991;216:3-10.
37. Sugiyama T, Okuma M, Ushikubi F, Sensaki S, Kanaji K, Uchino H. A novel platelet aggregating factor found in a patient with defective collagen-induced platelet aggregation and autoimmune thrombocytopenia. Blood 1987;69:1712-20.
38. Silva E, Souza J, Rogez H, Rees J, Larondelle Y. Antioxidant activities and polyphenolic contents of fifteen selected plant species from the Amazonian region. Food Chem 2007;101:1012-8.
39. Balamurugan E, Menon VP. In vitro radical scavenging activities of Chrysaora quinquecirrha nematocyst venom. Drug Discoveries Ther 2009;3:56-61.
40. Youn J, Hwang SH, Ryoo ZY, Lynes MA, Paik DJ, Chung HS, et al. Metallothionein suppresses collagen-induced arthritis via induction of TGF-beta and down-regulation of proinflammatory mediators. Clin Exp Immunol 2002;129:232-9.
41. Mendis E, Rajapakse N, Byun HG, Kim SK. Investigation of jumbo squid (Dosidicus gigas) skin gelatin peptides for their in vitro antioxidant effects. Life Sci 2005;77:2166-78.
42. Je JY, Qian ZJ, Byun HG, Kim SK. Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis. Process Biochem 2007;42:840-6.
43. Zhu KX, Zhou HM, Qian HF. Antioxidant and free radical scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase. Process Biochem 2006;41:1296-02.
44. Kim SK, Kim YT, Byun HG, Nam KS, Joo DS, Shahidi F. Isolation and characterization of anti-oxidative peptides from gelatin hydrolysate of Alaska Pollack skin. J Agric Food Chem 2001;49:1984-9.
45. Wu HC, Chen HM, Shiau CY. Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus). Food Res Int 2003;36:949-57.
46. Rajapakse N, Mendis E, Byun HG, Kim SK. Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. J Nut Biochem 2005;16:562-9.
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How to Cite
Hemavathi, M., P. Dhayananthan, S. Sivaranjani, S. Kumar, S. Shakthi, and C. Arulvasu. “ANTIOXIDANT AND CELL ADHESION PROPERTIES OF COLLAGEN FROM JELLYFISH ACROMITUS FLAGELLATUS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 9, July 2015, pp. 160-4, https://innovareacademics.in/journals/index.php/ijpps/article/view/7240.
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