ANTIOXIDANT AND HEPATOPROTECTIVE POTENTIALS OF STEMONOCOLEUS MICRANTHUS HARMS (FABACEAE) STEM BARK EXTRACT

Authors

  • F. N. Mbaoji Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, PMB 410001, Enugu State, Nigeria
  • A. C. Ezike
  • C. S. Nworu S. Nworu
  • C. A. Onyeto
  • I. A. Nwabunike
  • I. C. Okoli
  • P. A. Akah

Abstract

Objective: This study evaluated the antioxidant and hepatoprotective activities of the methanol-dichloromethane (1:1) extract of Stemonocoleus micranthus Harms (Fabaceae) stem bark (SME).

Methods: In vitro ferric reducing power, hydrogen peroxide and α, α-diphenyl–β–dipicryl–hydrazyl (DPPH) free radical scavenging assays, were used to determine the antioxidant activity of SME (25, 50, 100, 200 and 400 µg/ml). Also the effects of SME (100, 200 and 400 mg/kg) on liver enzymes alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) in carbon tetrachloride (CCl4)-inducedhepaticoxidative damage were studied in rats.

Results: The results showed that SME (25-400 µg/ml) significantly (P<0.01) reduced iron III (Fe3+) to iron II (Fe2+) with 400 µg/ml eliciting 135.4% reducing power. The SME demonstrated significant (P<0.01) hydrogen peroxide scavenging with 400 µg/ml eliciting 20.37% activity, comparable to ascorbic acid (20.32%). The SME (25-400 µg/ml) also elicited 77-81% DPPH free radical scavenging, lower than ascorbic acid (25-400 µg/ml) with 83-88% activity. The in vivo study showed that SME protected the rats from liver damage as shown by the reduction of liver enzymes in serum. The SME (400 mg/kg) elicited 7.7, 33.8 and 7.2% inhibition of ALP, ALT and AST respectively. The acute toxicity test revealed that SME has high margin of safety, with oral lethal dose (LD50)>5 g/kg. Phytochemical analyses on the extract revealed the presence of alkaloids, carbohydrates, flavonoids, glycosides, proteins, reducing sugars, saponins, resins, steroids, tannins, terpenoids, fats, and oils.

Conclusion: The findings suggest that the methanol-dichloromethane extract of S. micranthus stem bark possess antioxidant and hepatoprotective effects.

Keywords: Stemonocoleus micranthus, Antioxidant, Hepatoprotective, Albino rats, Ascorbic acid

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Published

01-07-2016

How to Cite

Mbaoji, F. N., A. C. Ezike, C. S. N. S. Nworu, C. A. Onyeto, I. A. Nwabunike, I. C. Okoli, and P. A. Akah. “ANTIOXIDANT AND HEPATOPROTECTIVE POTENTIALS OF STEMONOCOLEUS MICRANTHUS HARMS (FABACEAE) STEM BARK EXTRACT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 7, July 2016, pp. 47-51, https://innovareacademics.in/journals/index.php/ijpps/article/view/7493.

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