ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF LANNA MEDICINAL PLANTS USED IN MAHOOG FORMULA
Objectives: Antibacterial and antioxidant activities of Lanna medicinal plants used in Mahoog formula were investigated.
Methods: Dried powders of twenty five Lanna medicinal plants were extracted with ethanol using soxhlet's apparatus and with water by decoction method to obtain ethanolic and water extracts, respectively. Each extract was evaluated for antibacterial activity by agar diffusion technique and antioxidant activity by 2,2Â´-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical scavenging assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and ferric reducing antioxidant power (FRAP) assay.
Results: Most ofLanna medicinal plant extracts were active against gram-positive bacteria. The extract of Caesalpinia sappan (heart wood) showed the highest inhibitory effect on Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. Interestingly,the extract of Sindora siamensis (stem) exhibited potent activity against S. aureus as same as C. sappan with MIC and MBC values of 0.049 and 0.098 mg/ml, respectively. The antioxidant activities revealed that the extract of C. sappan and S. siamensis possess significant free radical scavenging and reducing power.
Conclusion: Most of the medicinal plants consisted in Mahoog formula revealed antioxidant and antibacterial activities. Thedata obtained from the study will be used as a scientific evidence to support the pharmacological properties of medicinal plants used in Mahoog formula.
2. Houghton PJ, Hylands PJ, Mensah AY, Hensel A, Deters AM. In vitro tests and ethnopharmacological investigations: wound healing as an example. J Ethnopharmacol 2005;100:100-7.
3. Srinivas RB, Reddy RKK, Naidu VG, Madhusudhana K, Agwane SB, Ramakrishna S, et al. Evaluation of antimicrobial, antioxidant and wound-healing potentials of Holoptelea integrifolia. J Ethnopharmacol 2008;115:249-56.
4. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.
5. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cationdecolorisation assay. Free Radical Biol Med 1999;26:1231-7.
6. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebenson Wiss Technol 1995;28:25â€“30.
7. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of â€˜â€˜antioxidant powerâ€™â€™: the FRAP assay. Anal Biochem 1996;239:70â€“6.
8. Kumarasamy Y, Cox PJ, Jaspars M, Nahar L, Sarker SD. Screening seeds of scottish plants for antibacterial activity. J Ethnopharmacol 2002;83:73-7.
9. Al-Fatimi M, Wurster M, Schroder G, Lindequist U. Antioxidant, antimicrobial and cytotoxic activities of selected medicinal plants from Yemen. J Ethnopharmacol 2007;111:657-66.
10. Kim KJ, Yu HH, Jeong SI, Cha JD, Kim SM, You YO. Inhibitory effects of Caesalpiniasappan on growth and invasion of methicillin-resistant Staphylococcus aureus. J Ethnopharmacol 2004;91:81-7.
11. Srinivasan R, Selvam GG, Karthik S, Mathivanan K, Baskaran R, Karthikeyan M, et al. In vitro antimicrobial activity of Caesalpinia sappan L. Asian Pac J Trop Biomed 2012;2:S136-9.
12. Elizabeth KM. Antimicrobial activity of Terminaliabellerica. Indian J Clin Biochem 2005;20:150-3.
13. Chowdhury JU, Bhuiyan NI, Nandi NC. Aromatic plants of Bangladesh: essential oils of leaves and fruits of Litsea glutinosa(Lour.) C. B. Robinson. Bangladesh J Bot 2008;37:81-3.
14. Badami S, Moorkoth S, Rai SR, Kannan E, Bhojraj S. Antioxidant activity of Caesalpiniasappan heartwood. Biol Pharm Bull 2003;26:1534-7.
15. Batubara I, Mitsunaga T, Ohashi H. Screening antiacne potency of Indonesian medicinal plants: antibacterial, lipase inhibition, and antioxidant activities. J Wood Sci 2009;55:230-5.
16. Wetwitayaklunga P, Phaechamudb T, Keokitichai S. The antioxidant activity of Caesalpinia sappanL. heartwood in various ages. Naresuan University J 2005;13:43-52.
17. Hu J, Yan X, Wang W, Wu H, Hua L, Du L. Antioxidant activity in vitro of three constituents from Caesalpinia sappan L. Tsinghua Sci Technol 2008;13:474-9.