IN VITRO ANTHELMINTIC EFFICACY OF ALPINIA NIGRA AND ITS BIOACTIVE COMPOUND, ASTRAGALIN AGAINST FASCIOLOPSIS BUSKI
Objective: Alpinia nigra (Zingiberaceae) is a traditionally used medicinal plant of North-east India against helminthiases. In view of its medicinal property the present study was designed to see the anthelmintic property of its major bioactive compound astragalin, compared to crude extract and praziquantel against Fasciolopsis buski.
Methods: Parasites were exposed to 2, 5, 10 and 20 mg/ml concentrations of plant extract and 0.025, 0.05 and 0.10 mg/ml concentration of astragalin in phosphate buffer saline. Paralyzed parasites were processed for histochemical, biochemical and morphological studies. Two important tegumental enzymes acid-and alkaline phosphatase were studies for its alterations in kinetic parameters on exposure to extracts and astragalin.
Results: Treated parasites revealed complete paralysis followed by death in a dose-dependent manner. Histochemical and biochemical studies on enzymes showed alterations in the activities. Morphologically, destruction and deformation were seen in the surface architecture of F. buski on exposure to different treatment. Acid-and alkaline phosphatase enzyme showed optimum activity at pH 6 and 9.5 respectively. The Vmax changed from 11.02 and 10.24 ÂµM/min/mg protein in control to as low as 6.98 and 6.54 ÂµM/min/mg tissue proteins in treated parasite for acid and alkaline phosphatase, respectively. Alterations in the Km values have also been noticed between the control and treated parasites.
Conclusion: The altered enzyme activities, tegumental architecture and kinetic parameters as observed under the influence of the plant extract and astragalin clearly indicate that the botanicals of the tested plant and its bioactive glycoside, astragalin acts as an anthelmintic agent against the tested fluke F. buski.
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