• Rajendra K. Patel Department of Pharmaceutical Analysis, K. B. Institute of Pharmaceutical Education and Research, Gandhinagar, Gujarat, India
  • Jigar B. Patel K. B. Institute of Pharmaceutical Education and Research
  • Priti D. Trivedi K. B. Institute of Pharmaceutical Education and Research


Objective: A simple spectrophotometric method was developed for the estimation of total alkaloids in Tinospora cordifolia M. and its formulations.

Methods: The method based on the formation of yellow colored complex by reaction between bromocresol green (BCG) and alkaloids in medicinal plants. A yellow colored complex forms is easily extractable by chloroform at pH 4.7.

Results: The method was linear in concentration range of 2-10 μg/ml with λmax at 415 nm. The LOD and LOQ were found to be 0.215 and 0.652 respectively. The intra-day and inter-day precision and accuracy were within the acceptable criteria (relative standard deviation<2.0% and accuracy within 100±2%).

Conclusion: The developed method is simple, precise and accurate and can be adopted for the routine quality control and standardization of plant materials containing alkaloids and its pharmaceutical products.


Keywords: Tinospora cordifolia M, Total alkaloids, Bromocresol green (BCG)


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How to Cite
Patel, R. K., J. B. Patel, and P. D. Trivedi. “SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF TOTAL ALKALOIDS IN THE TINOSPORA CORDIFOLIA M. AND ITS HERBAL FORMULATIONS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 11, Aug. 2015, pp. 249-51, https://innovareacademics.in/journals/index.php/ijpps/article/view/7622.
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