NOVEL SMART pH SENSITIVE CHITOSAN GRAFTED ALGINATE HYDROGEL MICROCAPSULES FOR ORAL PROTEIN DELIVERY: I. PREPARATION AND CHARACTERIZATION
Objectives: Preparation and characterization of a new pH sensitive chitosan (CS) grafted alginate (ALG) hydrogel microcapsules for the oral delivery of protein.
Methods: The pH sensitive hydrogel microcapsules were prepared for the first time using â€œgrafting toâ€ technique. Firstly, alginate was activated using Ï-Benzoquinone (PBQ) as a coupling agent to graft Chitosan chains later on. Both of activated and grafted alginate microcapsules were characterized by Fourier transform-Infra red spectroscopy (FT-IR), thermal gravimetric analysis (TGA) and the morphological structures were investigated using Scanning electron microscopy (SEM) examination.
Results: It was found that the optimum conditions affecting the activation process and also the swelling degree of the prepared hydrogel microcapsules were 2% ALG, 0.04M PBQ pH10, 45 Â°C for 2h. In addition, the grafting process depends on the attached amount of PBQ and CS concentration. Maximum grafting efficiency (GE %) and chitosan add-on percentage were 98.6% and 14.8% respectively using 0.3% CS at 40 Â°C for 3h.
Conclusions: Novel pH sensitive hydrogel microcapsules were prepared via grafting of chitosan molecules on to activated alginate backbone. The formulated microcapsules can be applied as a new pH sensitive carrier for protein drugs.
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