• Muchtaridi Muchtaridi Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl KM 21.5 Bandung-Sumedang, Jatinangor
  • Ester Yuliani, Iyan Sopyan Universitas Padjadjaran


Objective: The objective of this study is to determine the validity of analytical methods in OFX antibiotic study in human urine (in vitro) using an SPE-HPLC/UV. In this study, SPE was applied in preparing the analysis of ofloxacin using HPLC embedded UV detector.

Methods: C-18 (octadecylsilane) cartridge (100 mg, particle size 10 µm) of SPE was employed in preparing a sample to determine further of analytes using HPLC with phosphate buffer 0.025 M (pH 2.5) and acetonitrile (85.5:14.5) as mobile phase and a flow rate of 1.2 m l/min. UV detector was adjusted at 295 nm with the internal standard ciprofloxacin.

Results: The calibration curves for the ofloxacin were linear over concentrations ranging from 1.15 to 36.0 µg/ml with a correlation coefficient (r) from 0.9998 to 0.9999. The coefficients of variation obtained from ofloxacin were less than 10 %. Ofloxacin on the area ratio of peak height and a segment of the chromatogram, LOD and LOQ of ofloxacin were 0.12 and 0.4 μg/ml, respectively. The recovery of ofloxacin from spiked human urine was 96.0 %.

Conclusion: The validation methods that including parameters: selectivity, repeatability, linearity, detection limit, quantification limit, precision, accuracy, and suitability of the system. The methods used have validity according to the requirements that might be used to analyze ofloxacin in human urine.


Keywords: Ofloxacin, Solid Phase Extraction, HPLC


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How to Cite
Muchtaridi, M., and E. Y. I. Sopyan. “APPLICATION OFF-LINE SPE-HPLC/UV METHODS IN ANALYSIS OF OFLOXACIN IN HUMAN URINE (IN VITRO)”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 1, Nov. 2015, pp. 255-61,
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