• Sumana Dutta The University of Burdwan
  • Sanjib Ray Molecular Biology and Genetics Unit, Department of Zoology, The University of Burdwan, Golapbag, Burdwan 713104, West Bengal, India


Objective: To evaluate in vitro free radical scavenging, reducing power and total antioxidant activity of leaf extract fractions of Manilkara hexandra (Roxb.) Dubard in relation to their total phenolic contents.

Methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, Fe3+reducing power and the total antioxidant activities of the five extract fractions (petroleum ether extract fraction, PEF; chloroform extract fraction, CHF; ethyl acetate extract fraction, EAF; methanolic extract fraction, MEF; aqueous extract fraction, AQF) were performed and the results were expressed as ascorbic acid equivalent and the total phenolic content was also measured and the data expressed as tannic acid equivalent.

Results: Different extract fractions show the varied degree of free radical scavenging and reducing potentials and the order determined as MEF>AQF>EAF>CHF>PEF. The highest total antioxidant activity, 60.37±6.24 mg of ascorbic acid equivalent, and also the highest total phenolic content, 89.91±8.72 mg of tannic acid equivalent, were estimated in per gram of the leaf MEF. The MEF showed the maximum DPPH free radical scavenging activity, Fe3+reducing power and the total antioxidant activities than all the other extract fractions tested.

Conclusion: In vitro free radical scavenging and reducing potentials of the leaf extract fractions of M. hexandra were explored here and the leaf MEF identified as the most potential extract fraction contained the highest quantities of antioxidants and that positively correlate with the presence of relatively higher quantities of total phenolic contents.


Keywords: Antioxidant activity, Phenolics, DPPH, Total antioxidant


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1. Malik SK, Choudhary R, Kumar S, Dhariwal OP, Deswal RPS. Socio-economic and horticultural potential of Khirni [Manilkara hexandra (Roxb.) Dubard]: a promising underutilized fruit species of India. Genet Resour Crop Evol 2012;59:1255–65.
2. Warrier PK, Nambiar V PK, Ramankutty C. Indian medicinal plants a compendium of 500 species, volume 3, Orient Blackswan,-Material Medica; 1995.
3. Shah MB, Goswami SS, Santani DD. Effect of Manilkara hexandra (Roxb.) Dubard against experimentally-induced gastric ulcers. Phytother Res 2004;18:814–8.
4. Patel PR, Rao TVR. Screening of antibacterial activity of some underutilized fruits of Sapotaceae. Int Food Res J 2012;19:1227-31.
5. Parekh J, Chanda SV. Antibacterial activity of aqueous and alcoholic extracts of 34 Indian medicinal plants against some staphylococcus species. Turk J Biol 2008;32:63-71.
6. Nimbekar TP, Katolkar PP, Bhongade SL, Durugkar N. In vitro antioxidant activity of methanolic extract of Manilkara hexandra. J Adv Pharmacol Toxicol 2010;11:19-25.
7. Majewska M, Skrzycki M, Podsiad M, Czeczot H. Evaluation of antioxidant potential of flavonoids: an in vitro study. Acta Pol Pharm 2011;68:611-5.
8. Sies H. Oxidative stress, oxidants and antioxidants. Exp Physiol 1997;82:291-5.
9. Devasagayam TPA, Boloor KK, Ramsarma T. Methods for estimating lipid peroxidation: analysis of merits and demerits (minireview). Indian J Biochem Biophys 2003;40:300-8.
10. Vertuani S, Angusti A, Manfredini S. The antioxidants and pro-antioxidants network, an overview. Curr Pharm Des 2004;10:1677-94.
11. Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer, how are they linked? Free Radic Biol 2010;49:1603-16.
12. Noda Y, Anzai-Kmori A, Kohono M, Shimnei M, Packer L. Hydroxyl a superoxide anion radicalscavenging activities of natural source antioxidants using the computerized JES-FR30 ESR Spectrometer system. Int J Biochem Mol 1997;42:35-44.
13. Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 1998;46:4113-7.
14. Toma´s-Barbera´n FA, Espı´n J C. Phenolic compounds and related enzymes as determinants of quality in fruits and vegetables. J Sci Food Agric 2001;81:853-76.
15. Martinez-Tome M, Jimenez A, Ruggieri S, Frega N, Strabbioli R, Murcia M. Antioxidant properties of mediterranean spices compared with common food additives. J Food Prot 2001;64:1412–9.
16. Mohammedi Z, Atik F. Impact of solvent extraction type on total polyphenols Content and biological activity from Tamarix aphylla L. Karst. Int J Pharmacogn Biol Sci 2011;2:609-15.
17. Bravo L. Polyphenols, Chemistry, dietary sources, metabolism and nutritional significance. Nutr Rev 1988;56:317-33.
18. Pietta PG. Flavonoids as antioxidants journals of natural products Review. J Nat Prod 2000;63:1035-42.
19. Mandal SM, Chakraborty D, Dey S. Phenolic acids act as signaling molecules in plant–microbe symbioses. Plant Signaling Behav 2010;5:359-68.
20. Medina E, Brenes M, Romero C, Garcia A, De Castro A. A Main antimicrobial compounds in table olives. J Agric Food Chem 2007;55:9817-23.
21. Kaur GJ, Arora DS. Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complementary Altern Med 2009;30:1-10.
22. Martin KR, Appel CL. Polyphenols as dietary supplements; a double edged sword. Nutr Dietary Suppl 2010;2:1-12.
23. Servili M, Selvaggini R, Esposto S, Taticchi A, Montedoro G, Morozzi G. Health and sensory properties of virgin olive oil hydrophilic phenols, Agronomic and technological aspects of production that affect their occurrence in the oil. J Chromatogr A 2004;1054:113-27.
24. Brahmachari G. Bio-flavonoids with promising antidiabetic potentials. A critical survey. Oppor Challenge Scope Nat Prod Med Chem; 2011. p. 187-212.
25. Nijveldt RJ, Nood E, Hoorn DEC, Boelens PG, Norren KV, Paul AM. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr 2001;74:418–25.
26. Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D. Dietary antioxidant flavonoids and risk of coronary heart disease, the Zutphen Elderly Study. Lancet 1993;342:1007-11.
27. Dimitrios B. Sources of natural phenolic antioxidants. Trends Food Sci Tech 1996;7:505–12.
28. Dapkevicius A, Venskutonis R, Van Beek TA, Linssen JPH. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. J Sci Food Agric 1998;77:140-6.
29. Ayoola GA, Sofidiya T, Odukoya O, Coker HAB. Phytochemical screening and free radical scavenging activity of some nigerian medicinal plants. J Pharm Sci Pharm Pract 2008;8:133-6.
30. Brand-Willims W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995;28:25-30.
31. Stanković MS. Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac J Sci 2011;33:63-72.
32. Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986;44:307-15.
33. Garrat DC. The quantitative analysis of drugs. Japan: Chapman Hall; 1964. p. 456–8.
34. Harborne JB. Phytochemical methods Chapman and Hall, Ltd., London; 1973. p. 49-188.
35. Trease GE, Evans WC. Pharmacognosy Brailliar Tiridel can. 13th ed. Macmillian Publishers; 1989.
36. Sofowara A. Medicinal plants and Traditional medicine in Africa Spectrum Books Ltd., Ibadan, Nigeria; 1993. p. 289.
37. Dutta S, Ray S. Evaluation of antioxidant potentials of leaf aqueous and methanolic extracts of Calophyllum inophyllum in relation to total phenol and flavonoid contents. Int J Pharma Bio Sci 2014;5:441–50.
38. Makkar HPS, Blummel M, Borowy NK, Becker K. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. J Sci Food Agric 1993;61:161-5.
39. Kantamreddi VSSN, Lakshmi YN, Kasapu VVVS. Preliminary phytochemical analysis of some important indian plant species. Int J Pharma Bio Sci 2010;1:351-7.
40. Hatano T, Edamatsu R, Hiramatsu M, Mori A, Fujita Y. Effects of tannins and related polyphenols on superoxide anion radical and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem Pharm Bull 1989;37:2016-21.
41. Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, et al. Antioxidant activity of plant ext racts containing phenolic compounds. J Agric Food Chem 1999;47:3954-62.
42. Yen GC, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem 1995;43:27-32.
43. Khan RA. Evaluation of flavonoids and diverse antioxidant activities of Sonchus arvensis. Chem Cent J 2012;6:126.
44. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 2001;49:5165-70.
45. Beevi SS, Narasu M, Gowda BB. Polyphenolics profile, Antioxidant and radical scavenging activity of leaves and stem of Raphanus sativus L. Plant Foods Hum Nutr 2010;65:8–17.
46. Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, et al. Bioactive compounds in foods: their role in the prevention of cardioascular disease and cancer. Am J Med 2002;113:71–88.
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How to Cite
Dutta, S., and S. Ray. “EVALUATION OF IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF LEAF EXTRACT FRACTIONS OF MANILKARA HEXANDRA (ROXB) DUBARD IN RELATION TO TOTAL PHENOLIC CONTENTS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 11, Aug. 2015, pp. 296-01, https://innovareacademics.in/journals/index.php/ijpps/article/view/7989.
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