EVALUATION OF IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF LEAF EXTRACT FRACTIONS OF MANILKARA HEXANDRA (ROXB) DUBARD IN RELATION TO TOTAL PHENOLIC CONTENTS
Objective: To evaluate in vitro free radical scavenging, reducing power and total antioxidant activity of leaf extract fractions of Manilkara hexandra (Roxb.) Dubard in relation to their total phenolic contents.
Methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, Fe3+reducing power and the total antioxidant activities of the five extract fractions (petroleum ether extract fraction, PEF; chloroform extract fraction, CHF; ethyl acetate extract fraction, EAF; methanolic extract fraction, MEF; aqueous extract fraction, AQF) were performed and the results were expressed as ascorbic acid equivalent and the total phenolic content was also measured and the data expressed as tannic acid equivalent.
Results: Different extract fractions show the varied degree of free radical scavenging and reducing potentials and the order determined as MEF>AQF>EAF>CHF>PEF. The highest total antioxidant activity, 60.37Â±6.24 mg of ascorbic acid equivalent, and also the highest total phenolic content, 89.91Â±8.72 mg of tannic acid equivalent, were estimated in per gram of the leaf MEF. The MEF showed the maximum DPPH free radical scavenging activity, Fe3+reducing power and the total antioxidant activities than all the other extract fractions tested.
Conclusion: In vitro free radical scavenging and reducing potentials of the leaf extract fractions of M. hexandra were explored here and the leaf MEF identified as the most potential extract fraction contained the highest quantities of antioxidants and that positively correlate with the presence of relatively higher quantities of total phenolic contents.
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