COMPARATIVE CHEMOMETRIC PROFILES BETWEEN LEAF TISSUES OF WITHANIA SOMNIFERA CULTURED IN VITRO AND FIELD

Authors

  • Pankajavalli Thirugnanasambantham Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam University for Women, Coimbatore 641043, India
  • Kalaiselvi Senthil Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam University for Women, Coimbatore 641043, India
  • Taek-joo Oh College of Pharmacy, Chung-Ang University, Seoul 156756, Republic of Korea
  • Hyung Kyoon Choi College of Pharmacy, Chung-Ang University, Seoul 156756, Republic of Korea

Keywords:

Withania somnifera, In vitro leaf, Field grown, GC-MS, GABA, Putrescine

Abstract

Objective: Metabolomic profiling of herbal extracts is indispensable to standardize drugs and to inaugurate the scientific basis of their therapeutic properties. The present study was attempted with an objective to investigate a comparative GC-MS (Gas chromatography–Mass spectrometry) analysis of in vitro and field grown leaf tissues of “Indian ginseng”.

Methods: GC-MS often serves the methods of option for screening and quantitative metabolite profiling. In the present study, metabolic profiling of methanolic extracts of field and in vitro cultured Withania somnifera (Ashwagandha) leaf tissues were carried out using GC–MS technique.

Results: A total number of 39 primary metabolites in leaf were identified. These include alcohols, organic acids, purine, pyrimidine, sugars and putrescine. Highly significant qualitative and quantitative differences were noticed between the leaf tissues cultured in vitro and from the field. Especially, significant elevation in the accumulation of GABA (γ amino butyric acid) and putrescine was recorded in in vitro cultured leaf samples.

Conclusion: We conclude that in vitro cultures offers an intrinsic advantage to produce therapeutically valuable compounds, relatively in a short span of time and this principle determine its use as an alternative to field grown sample.

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Published

01-11-2015

How to Cite

Thirugnanasambantham, P., K. Senthil, T.- joo Oh, and H. K. Choi. “COMPARATIVE CHEMOMETRIC PROFILES BETWEEN LEAF TISSUES OF WITHANIA SOMNIFERA CULTURED IN VITRO AND FIELD”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 11, Nov. 2015, pp. 66-71, https://innovareacademics.in/journals/index.php/ijpps/article/view/8089.

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