ATOMIC FORCE MICROSCOPIC STUDY FOR THE ANTIBACTERIAL STUDY OF GARCINIA XANTHOCHYMUS HOOK. F. LEAF EXTRACT
Objective: The present study was undertaken to study the antibacterial effect of Garcinia xanthochymus Hook. f. (Clusiaceae), leaf extract against Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa using atomic force microscope.
Methods: Antibacterial study was done by disc diffusion and minimum inhibitory concentration. Atomic force microscopy study was carried to find out morphological changes in bacterial cells.
Results: Among the tested extracts (petroleum ether, chloroform and methanol), methanol extract inhibited the growth of Staphylococcus aureus, Bacillus cereus and Escherichia coli. The minimum inhibitory concentration varied from 25 to 50 Âµg/ml. The atomic force microscope was used to study the morphological alterations induced by the leaf extracts in S. aureus, B. cereus and E. coli. Reduction in the cell size, the formation of clusters, indicating the maximum damage in S. aureus when treated with leaf methanol extract, whereas cell rupture and surface roughness were observed in B. cereus and E. coli. P. aeruginosa was susceptible to leaf extracts of G. xanthochymus.
Conclusion: The methanol extract of Garcinia xanthochymus leaf extract was found to be the most effective antibacterial property against S. aureus, B. cereus and E. coli.
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