ANTI QUORUM SENSING POTENTIAL OF MORINGA OLEIFERA SEED EXTRACT
Objective: Most infectious diseases caused by bacteria are known to be regulated by Quorum-sensing mechanisms which in turn regulate the pathogenicity. Evaluation of phytochemicals present in Moringa plant extract for potential (QSI) Quorum sensing inhibitory activity was undertaken in this study.
Methods: QSI activity was evaluated using bioindicator organism Chromobacterium violaceum 12472. Biofilm dispersal activity of the QSI compound was evaluated by microtitre plate method. Individual bioactive components were extracted and analyzed by (TLC) Thin Layer Chromatography. Mechanism of the action of (MSE) Moringa seed extract was evaluated by LCMS analysis.
Results: Preliminary evaluation of different parts of Moringa oleifera extracts revealed that aqueous seed extract had shown a large turbid zone of violacein pigment inhibition when tested for Quorum sensing inhibitory activity with bioindicator organism Chromobacterium violaceum 12472 then the other plant parts. As the virulence factors like biofilm formation is regulated by Quorum sensing, biofilm dispersal activity was evaluated with selected bacterial pathogens. Biofilm dispersal activity was more in Pseudomonas (35%) and Klebsiella (25%) than the other pathogenic strains tested. Phytochemical components like saponins, cardiac glycosides, triterpenes, phytosterols and volatile oils were identified in aqueous Moringa Seed extract. After extraction and Thin Layer, Chromatography analysis only cardiac glycosides and saponins were found to have Quorum sensing inhibitory activity against Chromobacterium violaceum 12472.
Conclusion: In the present study the Quorum sensing inhibitory activity of crude Moringa Seed extract was observed to be more than the individual compounds like cardiac glycosides and saponins alone. Attempts were made to understand the mechanism involved in Quorum sensing inhibitory activity. Present results give an opportunity to explore the possible usage of Moringa Seed extract in treating biofilm formation by bacterial pathogen.
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