IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITY OF MIMOSA PUDICA LINN EXTRACT AND L-MIMOSINE ON LYMPHOMA DAUDI CELLS
Objective: The present study is an attempt to investigate the antioxidant and anticancer potential of hydroalcoholic extract of Mimosa pudica Linn (Mimosaceae) and L-Mimosine on Daudi cell line.
Methods: The analysis of the standard compound L-Mimosine was ascertained by High Performance Thin Layer Chromatography (HPTLC). Free radical scavenging activity of M. pudica extract and L-Mimosine was also compared using 2, 2-diphenylâ€“1-picrylhydrazylradical scavenging assay (DPPH). Cell viability and cytotoxicity on Daudi cells were evaluated by trypan blue and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays in a dose and time-dependent manner respectively.
Results: HPTLC analysis showed the presence of amino acids, amines, lipids in the hydroalcoholic extract of M. pudica. Crude hydroalcoholic extract of M. pudica showed antioxidant activity (IC50=103.88 Âµg/ml) whereas L-Mimosine showed antioxidant activity (IC50=233.06 ÂµM). The crude extract and compound inhibited the proliferation and growth of the Daudi cells through induced cell death. The IC50 value for anticancer activity was found to be 201.65 Âµg/ml and 86.61 ÂµM at 72 h for M. pudica extract and L-Mimosine respectively.
Conclusion: The results indicated the presence of L-Mimosine in the hydroalcoholic extract of M. pudica. The hydroalcoholic extract and pure compound proved potent inhibitors of cell proliferation, thus manifesting significant antioxidant and anticancer activity.
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