ISOLATION, SCREENING AND CHARACTERIZATION OF L-ASPARAGINASE PRODUCING FUNGI FROM MEDICINAL PLANTS

  • Ram Kumar Gulbarga University
  • Vijay Kumar Sedolkar Gulbarga University
  • A. G Triveni Gulbarga University
  • Mendem Suresh Kumar Gulbarga University
  • Channappa T. Shivannavar Gulbarga University
  • Subhaschandra M. Gaddad Department of Post Graduate Studies and Research in Microbiology, Gulbarga University, Kalaburagi, Karnataka 585106, India

Abstract

Objective: To isolate and characterize of L-asparaginase producing fungi from medicinal plants.

Methods: Fungal strains were isolated on standard media, characterized and were screened for their ability to produce L-asparginase, used in the treatment of certain types of cancers, using modified Czapek-Dox medium supplemented with L-asparagine.

Results: A total of 10 fungal isolates were obtained from 3 plant leaf samples and two isolates exhibited significant L-aspargniase production. Fusarium sp. (SMGR-F1) isolated from the papaya leaves showed the maximum activity. The organism was grown under submerged fermentation conditions at 30 °C produced 111.07±1.53 IU/ml of L-asparaginase at 120 h.

Conclusion: L-asparaginase is being effectively used for the treatment of acute lymphoblastic leukemia and tumor cells. Fusarium sp. was isolated from the papaya plant leaves showing significant L-asparaginase activity and thus can be further exploited for the commercial production of L-asparaginase.

 

Keywords: L-asparaginase, Fusarium sp. Papaya leaves, Submerged fermentation

Downloads

Download data is not yet available.

Author Biography

Subhaschandra M. Gaddad, Department of Post Graduate Studies and Research in Microbiology, Gulbarga University, Kalaburagi, Karnataka 585106, India

Department of Microbiology,

Professor

References

1. Verma N, Kumar K, Kaur G, Anand S. L-asparaginase: a promising chemotherapeutic agent. Crit Rev Biotechnol 2007;27:45-62.
2. Siddaligeshwara KG, Lingappa K. Production and characterization of L-asparaginase A tumour inhibitor. Int J PharmTech Res 2011;3:314-9.
3. Warangkar SC, Khobragade CN, Dawane BS, Bhosale RB. Effect of dihydropyridine deravatives on the kinetic parameter of E. caratovora L-asparaginase. Int J Biotechnol Appl 2009;1:5-13.
4. Tareke E, Rydberg P, Karlsson P, Eriksson S, Tornqvist M. Analysis of acrylamide, a carcinogen formed in heated foodstuffs. J Agric Food Chem 2002;50:4998–5006.
5. Sarquis MI, Oliveira EM, Santos AS, Costa GL. Production of L-asparaginase by filamentous fungi. Mem Inst Oswaldo Cruz 2004;99:489-92.
6. Lapmak K, Lumyong S, Thongkuntha S, Wongputtisin P, Sarsud U. L-asparaginase production by Biopolaris sp. BR 438 isolated from brown rice in Thailand. Chiang Mai J Sci 2010;37:160-4.
7. Rossi F, Incorvaia C, Mauro M. Hypersensitivity reactions to antineoplastic chemotherapeutic agents. Recenti Prog Med 2004;95:476-81.
8. Ramya LN, Doble M, Rekha VP, Pulicherla KK. L-asparaginase as potent anti-leukemic agent and its significance of having reduced glutaminase side activity for the better treatment of acute lymphoblastic leukemia. Appl Biochem Biotechnol 2012;167:2144-59.
9. Hyde KD, Soytong K. Understanding microfungal diversity-a critique. Cryptogam Mycol 2007;28:281-9.
10. Hyde KD, Soytong K. The fungal endophyte dilemma. Fungal Diversity 2008;33:163-73.
11. Gulati R, Saxena RK, Gupta R. A rapid plate assay for screening L-asparaginase producing micro-organisms. Lett Appl Microbiol 1997;24:23-6.
12. Imada A, Igarasi S, Nakahama K, Isono M. Asparaginase and glutaminase activities of micro-organisms. J Gen Microbiol 1973;76:85-99.
13. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
14. Aneja KR. Experiments in microbiology, plant pathology and biotechnology, New Age International (p). Ltd., Publishers. 4th edition. New Delhi; 2003.
15. Soniyamby Ambi Rani, Lalita S, Praveesh Bahuleyan Vasantha. Isolation and screening of l-asparaginase producing fungi from soil samples. Int J Pharm Pharm Sci 2012:5:426-7.
16. Niharika Yadav, Supriya Sarkar. Production of L-Asparaginase by Fusarium oxysporum using submerged fermentation”. Int J Pharm Sci Invent 2014;3:32-40.
17. Jayaramu M, Hemalatha NB, Rajeshwari CP, Siddalingeshwara KG, Mohsin SM. A novel approach for detection, confirmation, and optimization of L-asparaginase from Emericella nidulans. Curr Pharm Res 2010;1:20-4.
18. Rani SA, Lalitha S, Praveesh BV. In vitro antioxidant and anticancer activity of L-asparaginase from Aspergillus flavus (KUFS20). Asian J Pharm Clin Res 2011;4:174-7.
19. Mushtaq MS, Siddalingeshwara KG, Karthic J, Sunil DPLNSN, Naveen M, Prathiba KS. Rapid screening and confirmation of L-asparaginase from Penicillium sp. Int J Res Pharmacol Pharm 2012;1:147-50.
20. Hosamani R, Kaliwal BB. Isolation, molecular identification and optimization of fermentation parameters for the production of L-asparaginase, an anticancer agent by Fusarium equiseti. Int J Microbiol Res 2011;3:108-19.
Statistics
518 Views | 2371 Downloads
How to Cite
Kumar, R., V. K. Sedolkar, A. G. Triveni, M. S. Kumar, C. T. Shivannavar, and S. M. Gaddad. “ISOLATION, SCREENING AND CHARACTERIZATION OF L-ASPARAGINASE PRODUCING FUNGI FROM MEDICINAL PLANTS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 1, Nov. 2015, pp. 281-3, https://innovareacademics.in/journals/index.php/ijpps/article/view/8838.
Section
Original Article(s)