GC-MS ANALYSIS OF METHANOLIC EXTRACT OF LEAVES OF RHODODENDRON CAMPANULATUM
Objective: Rhododendron campanulatum is a native of high altitude and is known for its medicinal properties. The present study is aimed to identify the phytochemical constituents in the leaf extract of R. campanulatum using Gas Chromatography and Mass Spectroscopy (GC-MS).
Methods: The methanolic leaf extract was prepared using Accelerated Solvent Extraction system at room temperature and high pressure. Phytochemical screening of methanolic extract of R. campanulatum was performed using GCMS-QP2010 Plus (Shimadzu, Kyoto, Japan) and the spectrum was interpreted on the basis of the databases of National Institute Standard and Technology (NIST11LIB) and WILEY8LIB.
Results: The GC-MS analysis revealed the presence of 49 phytochemical compounds in the methanolic leaf extract. Baccharis oxide (9.99%), betuligenol (8%), alpha and beta-amyrin (7.38 and 2.64%), geranyl acetate (5.91%), (R)-(-)-14-methyl-8-hexadecyn-1-ol (5.19%) and phthalic acid (5.16%) were identified as major constituents.
Conclusion: The methanolic leaf extract of R. campanulatum contains various phyto-compounds of pharmaceutical and industrial importance.
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