ESTIMATION OF BACOSIDE-A IN BACOPA MONNIERI AERIAL PARTS USING TLC DENSITOMETRY

  • Rajesh Kumar Rayat-Bahra Institute of Pharmacy, Hoshiarpur, Punjab, India,
  • Tejpal Singh Rayat-Bahra Institute of Pharmacy, Hoshiarpur, Punjab, India
  • Deepak Kumar Department of Pharm. Sciences and Drug Research, Punjabi University, Patiala, Punjab, India
  • Mandeep Singh Rayat-Bahra Institute of Pharmacy, Hoshiarpur, Punjab, India
  • Sandeep Kaur Rayat-Bahra Institute of Pharmacy, Hoshiarpur, Punjab, India,
  • Rajeev Garg ASBASJSM College of Pharmacy, Bela, Ropar, Punjab, India

Abstract

Objective: To estimate bacoside A in Bacopa monnieri aerial parts using a validated TLC densitometric method.

Methods: The content of bacoside A was estimated using standardized procedures. A standard curve was prepared by applying different concentrations of the marker. The estimation of bacoside A was achieved on precoated TLC plate using solvent system, i.e., toluene: ethyl acetate: methanol: formic acid (3:3.5:2.5:1) followed by scanning at 225 nm. The developed method was validated for the parameters described in the ICH guidelines.

Results: The content of bacoside A was determined to be 1.13±0.019% w/w in B. monnieri aerial parts. The instrumental precision, repeatability, linearity range, correlation coefficient, intra-day precision, inter-day precision, limit of detection (LOD), limit of quantification (LOQ) and accuracy were found to be 0.61% CV, 0.87% CV, 150-900 ng, 0.998, 1.43% CV, 1.79% CV, 32 ng/spot, 92 ng/spot and 97.19±0.39% respectively.

Conclusion: The developed method was found to be simple, economic, accurate, precise, and can be utilized for the quantitative as well as qualitative estimation of bacoside A in Bacopa monnieri.

 

Keywords: Bacopa, Nootropic, Saponin, TLC densitometry

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Author Biographies

Rajesh Kumar, Rayat-Bahra Institute of Pharmacy, Hoshiarpur, Punjab, India,

Asst. Prof.

Deptt. of Pharmaceutics

Tejpal Singh, Rayat-Bahra Institute of Pharmacy, Hoshiarpur, Punjab, India

Student

Deptt. of Pharmaceutics

Deepak Kumar, Department of Pharm. Sciences and Drug Research, Punjabi University, Patiala, Punjab, India

Research Scholar

Punjabi University, Patiala

Mandeep Singh, Rayat-Bahra Institute of Pharmacy, Hoshiarpur, Punjab, India

Asst. Prof.

Rayat Bahra Institute of Pharmacy, Hoshiarpur

Sandeep Kaur, Rayat-Bahra Institute of Pharmacy, Hoshiarpur, Punjab, India,

Student

Deptt. of Pharmaceutics

Rajeev Garg, ASBASJSM College of Pharmacy, Bela, Ropar, Punjab, India

Asst. Prof.

Deptt. of Pharmaceutics

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How to Cite
Kumar, R., T. Singh, D. Kumar, M. Singh, S. Kaur, and R. Garg. “ESTIMATION OF BACOSIDE-A IN BACOPA MONNIERI AERIAL PARTS USING TLC DENSITOMETRY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 12, Oct. 2015, pp. 293-5, https://innovareacademics.in/journals/index.php/ijpps/article/view/8865.
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