COMPARATIVE PHARMACOGNOSTICAL AND PHYTOCHEMICAL STUDY OF VARIOUS SPECIES OF THE CONTROVERSIAL DRUG ‘PUNARNAVA'

  • Bhavika R Vala School of Pharmacy, RK University
  • Khyati R Bhalara School of Pharmacy, RK University
  • Devang J Pandya School of Pharmacy, RK University

Abstract

Objective: Punarnava is a controversial drug i.e. its true botanical identity has not been ascertained, and several different species are used as Punarnava. It is a part of many medicinally important traditional formulations. Ayurvedic Pharmacopoeia of India describes three different species–Roots of Boerhaavia diffusa, Trianthema portulacastrum and Boerhaavia verticillata. The present work deals with the microscopic, macroscopic and phytochemical comparison of the roots of these species of Punarnava, so as to differentiate them and establish their quality parameters.

Methods: Roots of these three species were collected, and their comparative morphological, microscopical (transverse section, powder study) and the phytochemical (screening, tannin estimation) study was performed.

Results: Roots of Boerhaavia diffusa showed morphological characteristics which clearly distinguished it from the other species. They showed the presence of well developed and stratified cork, abnormal vascular bundles which were discontinuous, and a central cavity, presumably formed due to the disintegration of tissues. Boerhaavia verticillata showed the presence of xylem bundles arranged in the form of a ‘X'-shaped cross in the central region. Thick-walled cork cells and pitted xylem vessels were present only in Boerhaavia diffusa root powder, whereas bundles of fibrovascular tissue were present only in the powder of Boerhaavia verticillata. Tannin content was found to be highest in Boerhaavia diffusa roots.

Conclusion: The present investigation will help herbal industries and traditional medicinal practitioners to detect adulteration of the medicinally important immunomodulator Boerhaavia diffusa by Trianthema portulacastrum and Boerhaavia verticillata, which are considered to have no immunomodulatory activity.

 

Keywords: Boerhaavia diffusa, Boerhaavia verticillata, Controversial drug, Immunomodulator, Microscopy, Nyctaginaceae, Punarnava, Quality Control, Standardization, Trianthema portulacastrum

Downloads

Download data is not yet available.

References

1. Anonymous. The Ayurvedic Pharmacopoeia of India. Part I. Vol. I. New Delhi: Ministry of AYUSH; 2007. p. 163-4.
2. Khare CP. Indian medicinal plants. Berlin: Springer; 2007. p. 96.
3. Anonymous. The Ayurvedic Pharmacopoeia of India. Part I. Vol. I. New Delhi: Ministry of AYUSH; 2007. p. 136-7.
4. Khare CP. Indian medicinal plants. Berlin: Springer; 2007. p. 668-9.
5. Anonymous. The Ayurvedic Pharmacopoeia of India. Part I. Vol. I. New Delhi: Ministry of AYUSH; 2007. p. 168-9.
6. Khare CP. Indian medicinal plants. Berlin: Springer; 2007. p. 96.
7. Khandelwal KR. Practical pharmacognosy techniques and experiments. Pune: Nirali Prakashan; 1996. p. 146-9.
8. Feigl F. Identification of the individual organic compound. In: Spot tests in organic analysis. London: Elsevier; 1956. p. 237-45.
9. Fishcher R. Praktikum der pharmakognosic. 3rd Ed. Berlin: Springer; 1952. p. 55-125.
10. Geissman A. Modern methods of plant analysis. Vol. III. Berlin: Springer; 1955. p. 75-80.
11. Harborne JB. Phytochemical methods. 2nd Ed. London: Chapman and Hall; 1973. p. 25-189.
12. Robinson T. The organic constituents of higher plants, their chemistry and interrelationships. Minneapolis: Burgers; 1964. p. 35-138.
13. Kanimozhi D, Kandhymathi K, Bharathidasan R, Mahalingam R, Deepa S, Panneerselvam A. Antioxidant activity, estimation of total phenolic content and tannin of Leucas aspera and Cassia auriculata. World J Sci Technol 2011;1:11-7.
Statistics
281 Views | 1946 Downloads
How to Cite
Vala, B. R., K. R. Bhalara, and D. J. Pandya. “COMPARATIVE PHARMACOGNOSTICAL AND PHYTOCHEMICAL STUDY OF VARIOUS SPECIES OF THE CONTROVERSIAL DRUG ‘PUNARNAVA’”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 1, Nov. 2015, pp. 297-01, https://innovareacademics.in/journals/index.php/ijpps/article/view/9129.
Section
Original Article(s)