THE ANTIâ€“ANGIOGENIC ACTIVITY OF PHOENIX DACTYLIFERA SEEDS EXTRACTS
Keywords:Angiogenesis, ex vivo study, Phoenix dactylifera Seeds
Objective: To identify the possible antiâ€“angiogenic activity of Phoenix dactylifera seeds extract.
Methods: The powder of the date palm seeds was extracted sequentially with petroleum ether, chloroform, methanol and water using the cold method "maceration" as extraction process. The ex vivo rat aorta ring assay was used to screen the extracts for possible antiâ€“angiogenesis activity, this assay was also used to determine the doseâ€“response effect of the active extract(s) by preparing serial concentrations. Free radical scavenging activity of the active extract(s) was determined using DPPH (1, 1â€“diphenylâ€“2â€“picrylhydrazyl) assay.
Results: The obtained data revealed that the four extracts exhibited significant inhibition of blood vessels growth when they were compared to the negative control (received DMSO 1%) (P<0.001), but chloroform and methanol extracts showed the highest percent of inhibition of blood vessels growth. According to the screening results, both chloroform and methanol extracts were selected for further investigation. Each of chloroform and methanol extracts of Phoenix dactylifera seeds exhibited a significant doseâ€“dependent antiâ€“angiogenesis effect with IC50 (30.9 Âµg/ml and 28.4 Âµg/ml) respectively. Furthermore, chloroform and methanol extracts exhibited a significant free radical scavenging activity (P<0.05) with IC50 (81.02 Âµg/ml and 16.33 Âµg/ml) respectively.
Conclusion: the results revealed that each of chloroform and methanol extracts of phoenix dactylifere seeds exhibited the best and most significant antiâ€“angiogenesis activity as well as a significant free radical scavenging activity.
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