THE ANTI–ANGIOGENIC ACTIVITY OF PHOENIX DACTYLIFERA SEEDS EXTRACTS

  • Adeeb A Al-zubaidy Al-Nahrain University
  • Hayder B Sahib Al-Nahrain University/College of Pharmacy/Pharmacology Department
  • Zeena A Hussein Al-Nahrain University

Abstract

Objective: To identify the possible anti–angiogenic activity of Phoenix dactylifera seeds extract.

Methods: The powder of the date palm seeds was extracted sequentially with petroleum ether, chloroform, methanol and water using the cold method "maceration" as extraction process. The ex vivo rat aorta ring assay was used to screen the extracts for possible anti–angiogenesis activity, this assay was also used to determine the dose–response effect of the active extract(s) by preparing serial concentrations. Free radical scavenging activity of the active extract(s) was determined using DPPH (1, 1–diphenyl–2–picrylhydrazyl) assay.

Results: The obtained data revealed that the four extracts exhibited significant inhibition of blood vessels growth when they were compared to the negative control (received DMSO 1%) (P<0.001), but chloroform and methanol extracts showed the highest percent of inhibition of blood vessels growth. According to the screening results, both chloroform and methanol extracts were selected for further investigation. Each of chloroform and methanol extracts of Phoenix dactylifera seeds exhibited a significant dose–dependent anti–angiogenesis effect with IC50 (30.9 µg/ml and 28.4 µg/ml) respectively. Furthermore, chloroform and methanol extracts exhibited a significant free radical scavenging activity (P<0.05) with IC50 (81.02 µg/ml and 16.33 µg/ml) respectively.

Conclusion: the results revealed that each of chloroform and methanol extracts of phoenix dactylifere seeds exhibited the best and most significant anti–angiogenesis activity as well as a significant free radical scavenging activity.

 

Keywords: Angiogenesis, ex vivo study, Phoenix dactylifera Seeds

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References

1. Hayder B Sahib, Adeeb A Al-Zubaidy, Shallal M Hussain, Ghaith Ali Jassim. The anti-angiogenic activity of Vitex agnus castus leaves extracts Int J Pharm Pharm Sci 2014;6:863-9.
2. Hayder B Sahib, Aisha AF, Yam Mun Fe, Ismail Z, Asmawi Z, Salhimi SM, et al. Anti-angiogenic and anti-oxidant properties of Orthosiphon stamineus Benth. methanolic leaves extract. Int J Pharm 2009;5:162-7.
3. Gerhardt H. VEGF and endothelial guidance on angiogenic sprouting. Organogen 2008;4:241-6.
4. Djonov VG, Baum O, Burri PH. Vascular remodeling by intussusceptive angiogenesis. Cell Tissue Res 2003;314:107-17.
5. Carmeliet P. Angiogenesis in health, disease and medicine. Nat 2005;438:932-6.
6. Zaid A, Dewet PF. Date palm cultivation. Botanical and systematic description of the date palm. FAO 2002;156:1.
7. Chao CT, Robert RK. The date palm (Phoenix dactylifera L.): Overview of biology, uses, and cultivation. Hort Sci 2007;42:1077-82.
8. Devshony S, Eteshola A, Shani A. Characterization and some potential application of date palm (Phoenix dactylifera L.) seeds and seeds oil. J Am Oil Chem Soc 1992;69:595-7.
9. Al-Farsi MA, Lee CY. Optimization of phenolics and dietary fiber extraction from date seeds. Food Chem 2008;108:977-85.
10. Brown K, Maynes S, Bezos A, Maguire D, Ford M, Parish C. A novel in vitro assay for human angiogenesis. Lab Invest 1996;75:539-55.
11. Nicosia RF. The aortic ring model of angiogenesis: a quarter century of search and discovery. J Cell Mol Med 2009;13:4113-36.
12. Janet sw, Judith A Boice, J Anthony MF, Matt SA. An ex vivo angiogenesis assay utilizing commercial porcine carotid artery: Modification of the rat aortic ring assay. Angiogenesis 2001;1:3-43.
13. Silvia B, Laetitia D, Agnes N, Jean-MF. Quantification of angiogenesis on the rat aortic ring assay. Image Anal Stereol 2003;22:43-8.
14. Das K, Tiwari RKS, Shrivastava DK. Techniques for evaluation of medicinal plant products as antimicrobial agent: current methods and future trends. J Med Plants Res 2010;4:104-11.
15. Widsten P, Laine JE, Qvintus-Leino P, Tuominen S. Effect of high-temperature defibration on the chemical structure of hardwood. Holzforschung 2002;56:51-9.
16. Prashant T, Bimlesh K, Mandeep K, Gurpreet K, Harleen K. Phytochemical screening and extraction: a review. Int Pharm Sci 2011;1:98-106.
17. Nicosia RF, Lin YJ, Hazelton D, Qian X. Endogenous regulation of the angiogenesis in the rat aorta model. Role of vascular endothelial growth factor. Am J Pathol 1997;151:115-22.
18. Battinelli EM, Markens BA, Italiano JE Jr. The release of angiogenesis regulatory proteins from platel et al. pha granules: modulation of physiologic and pathologic angiogenesis. Blood 2011;118:1359–69.
19. Kadioglu O, Seo EJ, Efferth T. Targeting angiogenesis by phytochemicals. Med Aromat Plants 2013;2:100-34.
20. Al–Rawahi AS, Rahman MS, Guizani N, Essa MM. Chemical composition, water sorption isotherm, and phenolic contents in fresh and dried pomegranate peels. Drying Technol 2013;31:257–63.
21. Shanmugam MK, Dai X, Kumar AP, Tan BK, Sethi G, Bishayee A. Oleanolic acid and its synthetic derivatives for the prevention and therapy of cancer: preclinical and clinical evidence. Cancer Lett 2014;346:206–16.
22. Pan YM, Zhu JC, Wang HS, Zhang XPY, He CH, Ji XW, et al. Antioxidant activity of ethanolic extract of Cortex fraxini and use in peanut oil. Food Chem 2007;103:913-8.
23. Wang Y, Zang QS, Liu Z, Wu Q, Maass D, Dulan G, et al. Regulation of VEGF-induced endothelial cell migration by mitochondrial reactive oxygen species. Am J Physiol: Cell Physiol 2011;301:695-704.
24. Mikirova NA, Ichim TE, Riordan NH. Anti-angiogenic effect of high doses of ascorbic acid. J Transl Med 2010:6:50-60.
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How to Cite
Al-zubaidy, A. A., H. B. Sahib, and Z. A. Hussein. “THE ANTI–ANGIOGENIC ACTIVITY OF PHOENIX DACTYLIFERA SEEDS EXTRACTS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 1, Nov. 2015, pp. 311-5, https://innovareacademics.in/journals/index.php/ijpps/article/view/9213.
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