• Tengku Siti Hajar Haryuna Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan 20155, Indonesia
  • Wibi Riawan Faculty of Medicine, Universitas Brawijaya
  • Muhammad Reza Faculty of Medicine, Universitas Brawijaya
  • Nyilo Purnami Faculty of Medicine, Universitas Brawijaya
  • Adlin Adnan Faculty of Medicine, Universitas Brawijaya


Objective: To demonstrate curcumin as the safe and effective therapeutic agent in the prevention and treatment of oxidative damage in fibroblasts within the cochlear supporting tissues and lateral wall following noise exposure by neutralizing oxidative stress-inducing agents, such as malondialdehyde (MDA) and hydrogen peroxide (H2O2).

Methods: Twenty-four Rattus norvegicus were randomly divided into 4 groups (n = 6). Group 1: The control group; group 2: noise (+); group 3: noise (+), 50 mg/day curcumin (+); group 4: noise (+), 100 mg/day curcumin (+). All groups (except group 1) were subjected to 100 dB SPL for 2 h per day for 14 d. Curcumin used in this study was derived from Curcuma longa L. (Turmeric) with curcumin [28.1±1.0]% w/w compared to Standard and administered orally for 14 d. All samples were Immuno histo-chemistrically examined for the expressions of MDA in cochlear fibroblasts and colorimetrically examined for H2O2 levels in cochlear tissues using the colorimetric reader.

Results: The results obtained showed significant differences for the expressions of MDA (P<0.05) in all groups, and significant differences for H2O2 levels (P<0.05) in all groups, except in group 1 compared to 4 and group 3 compared to 4.

Conclusion: Curcumin proved to be potentially effective in the prevention and treatment of oxidative damage in fibroblasts within the cochlear supporting tissues and lateral wall following noise exposure by decreasing the expressions of MDA and H2O2 levels.


Keywords: Curcumin, Malondialdehyde, Hydrogen peroxide, Cochlea, Fibroblast, Antioxidant


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1. Nandi SS, Dhatrak SV. Occupational noise-induced hearing loss in India. Indian J Occup Environ Med 2008;12:53-6.
2. Khan HU, Ali SI. Evaluation of road traffic noise pollution in quetta (Pakistan). Am J Mod Phys 2014;3:29-36.
3. Tan WJT, Thorne PR, Vlajkovic SM. Noise-induced cochlear inflammation. World J Otorhinolaryngol 2013;3:89-99.
4. Wong ACY, Froud KE, Hsieh YSY. Noise-induced hearing loss in the 21st century: A research and translational update. World J Otorhinolaryngol 2013;3:58-70.
5. Mates JM. Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. Toxicology 2000;153:83-104.
6. Rahman K. Studies on free radicals, antioxidants, and co-factors. Clin Interventions Aging 2007;2:219-36.
7. Khalil S, Awad A, Elewa Y. The antidotal impact of extra virgin olive oil against genotoxicity, cytotoxicity and immunotoxicity induced by hexavalent chromium in rat. Int J Veterinary Sci Med 2013;1:65-73.
8. Poirrier AL, Pincemail J, Van den Ackerveken P, Lefebvre PP, Malgrange B. Oxidative stress in the cochlea: an update. Curr Med Chem 2010;17:1-14.
9. Rewerska A, Pawelczyk M, Rajkowska E, Politanski P, Sliwinska-Kowalska M. Evaluating D-methionine dose to attenuate oxidative stress-mediated hearing loss following overexposure to noise. Eur Arch Oto-Rhino-Laryngol 2013; 270:1513-20.
10. Trujillo J, Granados-Castro LF, Zazueta C, Anderica-Romero AC, Chirino YI, Pedraza-Chaverri J. Mitochondria as a target in the therapeutic properties of curcumin. Arch Pharm Chem Life Sci 2014;347:873-84.
11. Yadav SK, Sah AK, Jha RK, Sah P, Shah DK. Turmeric (curcumin) remedies gastroprotective action. Pharmacogn Rev 2013;7:42-6.
12. Sall J, Lehman A, Stephens M, Creighton L. JMP start statistics: a guide to statistics and data analysis using JMP. 5th ed. USA: SAS Institute; 2012.
13. Bielefeld EC, Hu BH, Harris KC, Henderson D. Damage and threshold shift resulting from cochlear exposure to paraquat-generated superoxide. Hear Res 2005;207:35-42.
14. McFadden SL, Ohlemiller KK, Ding D, Shero M, Salvi RJ. The influence of superoxide dismutase and glutathione peroxidase deficiencies on noise are¬ induced hearing the loss in mice. Noise Health 2001;3:49-64.
15. Mukherjea D, Rybak LP, Sheehan KE, Kaur T, Ramkumar V, Jajoo S, et al. The design and screening of drugs to prevent acquired sensorineural hearing loss. Expert Opin Drug Discovery 2011;6:491-505.
16. Wong ACY, Ryan AF. Mechanisms of sensorineural cell damage, death and survival in the cochlea. Front Aging Neurosci 2015;7:1-15.
17. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010;4:118-26.
18. Jebakumar AZ, Nondo HS, George SK, Manoj G. Natural anti-oxidants and in-vitro methods for antioxidant activity. Int J Pharmacol Res 2012;2:46-55.
19. Nimse SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv 2015;5:27986-8006.
20. Li X, Fang P, Mai J, Choi ET, Wang H, Yang X. Targeting mitochondrial reactive oxygen species as a novel therapy for inflammatory diseases and cancers. J Hematol Oncol 2013,6:1-19.
21. Krishnamurthy P, Wadhwani A. Antioxidant enzymes and human health. In: El-Missiry MA. editor. Antioxidant enzyme. 1st ed. Rijeka: InTech; 2012. p. 3-18.
22. Grotto D, Maria LS, Valentini J, Paniz C, Schmitt G, Garcia SC, et al. Importance of the lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantification. Quim Nova 2009;32:169-74.
23. Repetto M, Semprine J, Boveris A. Lipid peroxidation: Chemical mechanism, biological implications and analytical determination. In: Catala A. editor. Lipid peroxidation. 1st ed. Rijeka: InTech; 2012. p. 3-30.
24. Derekoy FS, Dundar Y, Aslan R, Cangal A. Influence of noise exposure on antioxidant system and TEOAEs in rabbits. Eur Arch Oto-Rhino-Laryngol 2001;258:518-22.
25. Yildirim I, Kilinc M, Okur E, Inanc TF, Kilic MA, Kurutas EB, et al. The effects of noise on hearing and oxidative stress in textile workers. Ind Health 2007;45:743-9.
26. Dehghani A, Ranjbarian M, Khavanin A, Azari MR, Vosooghi S. Exposure to noise pollution and its effect on oxidant and antioxidant parameters in blood and liver tissue of rat. Zahedan J Res Med Sci 2013;15:13-7.
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How to Cite
Haryuna, T. S. H., W. Riawan, M. Reza, N. Purnami, and A. Adnan. “CURCUMIN PREVENTS COCHLEAR OXIDATIVE DAMAGE AFTER NOISE EXPOSURE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 1, Nov. 2015, pp. 175-8,
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