THE PRELIMINARY PHYTOCHEMICAL ANALYSIS AND ORAL ACUTE TOXICITY STUDY OF STEM BARK OF SYZYGIUM CUMINI
Keywords:Toxicity study, Syzygium cumini, Stem bark, Phytochemicals
Objective: The current subject field was carried out to evaluate phytochemical analysis and acute toxicity study of the stem bark of Syzygium cumini.
Methods: The ethanol extraction was made by the Soxhlet extraction method. Phytochemical screening was performed by applying standard methods. The oral administration of the drug in treating a group is done by using a curved ball-tipped intubation needle affixed to a 3 ml syringe and animal's observed from cage side observations. All the animals were anesthetized with ether anesthesia; blood was collected from orbital puncture and analysis was made by standard methods. Several organs were collected by dissecting abdominal, thoracic and cranial cavities and each weighed by using a sensitive electronic balance. Fixation was made with 10% buffered formalin and processed for histological embedding by Carleton's histological techniques. Statistical data analyzed by statistical software Sigma Plot 10.
Results: The phytochemical screening revealed the presence of flavonoids, tannins, carbohydrates, sterols and amino acids. The oral acute toxicity study showed no detectable clinical signs of toxicity and mortality during the study period of 14 d. There were no statistically significant alterations in body weights, organ weights, and hematological parameters compared to control group. The microscopic anatomy of the reproductive organs and liver showed normal architecture.
Conclusion: The outcome of the study showed that the LD50 of the drug was greater than 5000 mg/kg body weight. The phytochemicals present in stem bark of Syzygium cumini can be employed in the fabrication of innovative medication for several diseases.
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