IN VITRO CYTOTOXIC ACTIVITY OF CLERODENDRUM INFORTUNATUM L. AGAINST T47D, PC-3, A549 AND HCT-116 HUMAN CANCER CELL LINES AND ITS PHYTOCHEMICAL SCREENING

  • Mir Haris Kuvempu University
  • Riaz Mahmood Department of Biotechnology and Bioinformatics, Kuvempu University, Shimoga 577451, Karnataka, India
  • Haseebur Rahman Kuvempu University
  • Nazneen Rahman Kuvempu University

Abstract

Objective: Present investigation accounts for scientific evaluation of the plant Clerodendrum infortunatum L. for its medicinal efficacy which includes phytochemical screening and anticancer activities.

Methods: Phytochemical screening of Clerodendrum infortunatum extracts was performed for the qualitative detection of reducing sugars, terpenoids, flavonoids, saponins, tannins, alkaloids, phlobatannins, steroids, amino acids and glycosides using standard procedures. The sulforhodamine B (SRB), in vitro cytotoxic assay, was used to investigate the anticancer activity of hexane, chloroform, ethyl acetate and ethanol extracts of leaves and roots of Clerodendrum infortunatum against T47D (Breast), PC-3 (prostate), A549 (lung) and HCT-116 (colon) cancer cell lines.

Results: Secondary metabolites including alkaloids, flavonoids, terpenoids, steroids, tannins, and saponins are present in many extracts. Alkaloids and flavonoids found to be present in almost all the extracts. The best cytotoxic activity has been exerted by hexane of root exhibiting growth inhibition of 72.83 ± 0.44, 85.50 ± 0.29 and 68.17 ± 1.36 % against PC-3, A549 and HCT-116 at a concentration of 100 µg/ml. Further, hexane of root showed a moderate cytotoxic effect of 42.17 ± 0.17 % against T47D at 100 µg/ml. At similar concentration, the chloroform extract is also effective against these three cell lines showing 61.50 ± 0.76, 67.00±0.58, and 68.53 ± 0.80% growth inhibition against PC-3, A549 and HCT-116 cell lines respectively, whereas, T47D cancer cell line showed 46.43 ± 0.30 % growth inhibition. The results have indicated that all the leaf extracts, as well as ethyl acetate and ethanol extracts of root, have exhibited a poor response (≤ 40 %).

Conclusion: The present ï¬ndings suggest that the Clerodendrum infortunatum extracts are rich in alkaloid, flavonoids and terpenoids. The hexane and chloroform root extracts of Clerodendrum infortunatum possess signiï¬cant anticancer activities which may be due to the presence of these phytochemical groups.

 

Keywords: Alkaloids, Cancer cell lines, Clerodendrum infortunatum, Flavonoids, Growth inhibition

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References

1. Gennari C, Castoldi D, Sharon O. Natural products with taxol-like anti-tumor activity: synthetic approaches to eleuthero bin and dicty statin. Afr J Pure Appl Chem 2007;79:173-80.
2. Chhajed M, Sowle A, Baraskar R, Parihar N. Buserelin as a wonder drug for prostate cancer: a comprehensive study. Int J Pharm Res Sci 2012;1:150-72.
3. Rao MRP, Adagale UR, Shetty A, Namjoshi P, Gaitonde P, Jain P. Cancer Immunotherapy; 2007. Available from: http://www. pharmainfo.net/ reviews/cancer-immunotherapy. [Last accessed on 20 Sep 2015].
4. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin 2011;61:69-90.
5. Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 2010;15:7313-52.
6. Sporn MB, Liby KT. Cancer chemoprevention: scientific promise, clinical uncertainty. Nat Clin Pract Oncol 2005;2:518–25.
7. Gopal N, Sengottuvelu S. Hepatoprotective activity of Clerodendrum inerme against CCl4 induced hepatic injury in rats. Fitoterapia 2008;79:24-6.
8. Vidya SM, Krishna V, Manjunatha BK, Mankani KL, Ahmed M, Singh SD. Evaluation of hepatoprotective activity of Clerodendrum serratum L. Indian J Exp Biol 2007;45:538-42.
9. Chae S, Kim JS, Kang KA, Bu HD, Lee Y, Seo YR, et al. Antioxidant activity of iso acteoside from Clerodendrontrichotomum. J Toxicol Environ Health Part A 2005;68:389-400.
10. Chopra RN, Nayer SL, Chopra IC. Glossay of Indian medicinal plant. (Publication and Information Directorate, CSIR, New Delhi; 1992. p. 71.
11. Akihisa T, Matsubara Y, Ghosh P, Thakur S, Shimizu N, Tamura T, et al. The 24a-and 24ß-epimers of 24-ethylcholesta-5, 22-dien-3ß-ol in two Clerodendrum species. Phytochemistry 1988;27:1169-72.
12. Sinha NK, Seth KK, Pandey VB, Dasgupta B, Shah A. Flavonoids from the flower of Clerodendrum infortunatum. Planta Med 1981;42:296-8.
13. Sannigrahi S, Mazumder UK, Pal DK, Parida S. In vitro antioxidant activity of methanol extract of Clerodendrum infortunatum Linn. Oriental Pharm Exp Med 2009;9:128-34.
14. Rajakaruna N, Harris CS, Towers GHN. Antimicrobial activity of plants collected from serpentine outcrops in Srilanka. Pharm Biol 2002;40:235-44.
15. Goswami A, Dixit VK, Srivastava BK. Anti-malarial activity of an aqueous extract of Clerodendrum infortunatum. Bionature 1998;48:45-8.
16. Haris M, Riaz M, Haseeb R, Nazneen. Paralysis and death of Pheretima posthuma and fungal growth inhibition by leaf extracts of Clerodendrum infortunatum L. J Pharmacogn Phytochem 2015;3:188-92.
17. Pal DK, Sannigrahi S, Mazumder UK. Analgesic and anticonvulsant effects of saponin isolated from the leaves of Clerodendrum infortunatum Linn. in mice. Indian J Exp Biol 2009;47:743-7.
18. Mehta K, Patel BN, Jain BK. Phytochemical analysis of leaf extracts of Phyllanthusfraternus. Res J Recent Sci 2013;2:12-5.
19. Ashok Kumar, Jha KK, Dinesh Kumar, Abhirav Agarwal, Akhil Gupta. Preliminary phytochemical analysis of leaf and bark (Mixture) extracts of Ficusinfectoria Plant. Pharma Innovation 2012;1.5:71-6.
20. Sharma PR, Mondhe DM, Muthiah S, Pal HC, Shahi AK, Saxena AK. Anticancer activity of essential oil from Cymbopogonflexuosus. Chem Biol Interact 2009;179:160-8.
21. Samanta S, Pain A, Dutta S, Saxena AK, Shanmugavel M. Antitumor activity of nitro naphthol-NU, a novel mixed function agent. J Exp Ther Oncol 2005;5:15-22.
22. Houghton P, Fang R, Techatanawat I, Steventon G, Hylands PJ, Lee CC. The sulphorhodamine (SRB) assay and other approaches to testing plant extracts and derived compounds for activities related to the reputed anticancer activity. Methods 2007;42:377-87.
23. World Health Organization. The Global Burden of Disease. Cambridge, MA, USA: Harvard; 2008.
24. Schwartsmann G. Breast cancer in South America: challenges to improve early detection and medical management of a public health problem. J Clin Oncol 2011;19:118-24.
25. Zulak K, Liscombe D, Ashihara H, Facchini P. Alkaloids. Plant secondary metabolism in diet and human health. Oxford: Blackwell Publishing; 2006. p. 102–36.
26. Sharma DK. Pharmacological properties of flavonoids from plants. Am J Sci Ind Res 2006;65:477-84.
27. Li YW, Shao L, Hu BM6. A new semi-synthetic Vinca alkaloid exhibits its potent in vivo antitumor activities via its high binding affinity for tubulin and improved pharmacokinetic profiles.” Cancer Biol Ther 2007;65:787–94.
28. Huang M, Gao H, Chen Y. Chimmitecan, a novel 9-substituted camptothecin, with improved anticancer pharmacologic profiles in vitro and in vivo,” Clin Cancer Res 2007;13:1298–307.
29. Sun Y, Xun K, Wang Y, Chen X. A systematic review of the anticancer properties of berberine, a natural product from Chinese herbs. Anti-Cancer Drugs 2009;20:757–69.
30. Eom KS, Kim HJ, So HS, Park R, Kim TY. Berberine-induced apoptosis in human glioblastoma T98G Cells Is mediated by endoplasmic reticulum stress accompanying reactive oxygen species and mitochondrial dysfunction. Biol Pharm Bull 2010;33,10:1644–9.
31. Burgeiro A, Gajate C, Dakir EH, Villa-Pulgar JA, Oliveira PJ, Mollinedo F. Involvement of mitochondrial and B-RAF/ERK signaling pathways in berberine-induced apoptosis in human melanoma cells. Anti-Cancer Drugs 2011;22:507–18.
32. Shyu KG, Lin S, Lee CC. Evodiamine inhibits in vitro angiogenesis: implication for anti–tumorgenicity. Life Sci 2006;78:2234–43.
33. Zhang Y, Wu LJ, Tashiro SI, Onodera S, Ikejima T. Intracellular regulation of evodiamine-induced A375-S2 cell death. Biol Pharm Bull 2003;26:1543–7.
34. Ji YB. Active Ingredients of Traditional Chinese Medicine: Pharmacology and Application, People’s Medical Publishing Hourse Cp., LTD; 2011.
35. Dai ZJ, Gao J, Ji ZZ. Matrine induces apoptosis in gastric carcinoma cells via alteration of Fas/FasL and activation of caspase-3. J Ethnopharmacol 2009;123:91–6.
36. Ren W. Flavonoids: promising anticancer agents. Med Res Rev 2003;23:51934.
37. Pouget C, Lauthier F, Simon A, Fagnere C, Basly JP, Delage C, et al. Flavonoids: structural requirements for antiproliferative activity on breast cancer cells. Bioorg Med Chem Lett 2001;11:3095–7.
38. Han D, Tachibana H, Yamada K. Inhibition of the environmental estrogen-induced proliferation of human breast carcinoma MCF-7 cells by flavonoids. In Vitro Cell Dev Biol: Anim 2001;37:275–82.
39. Bai F, Matsui T, Ohtani-Fujita N, Matsukawa Y, Ding Y, Sakai T. Promoter activation and following induction of the p21/WAF1 gene by flavone is involved in G1 phase arrest in A549 lung adenocarcinoma cells. FEBS Lett 1998;437:61–4.
40. Caltagirone S, Ranelletti FO, Rinelli A, Maggiano N, Colasante A, Musiani P, et al. Interaction with type II estrogen binding sites and antiproliferative activity of tamoxifen and quercetin in human non-small-cell lung cancer. Am J Respir Cell Mol Biol 1997;17:51–9.
41. Knowles LM, Zigrossi DA, Tauber RA, Hightower C, Milner JA. Flavonoids suppress androgen independent human prostate tumor proliferation. Nutr Cancer 2000;38:116–22.
42. Kampa M, Hatzoglou A, Notas G, Damianaki A, Bakogeorgou E, Gemetzi C, et al. Wine antioxidant polyphenols inhibit the proliferation of human prostate cancer cell lines. Nutr Cancer 2000;37:223–33.
43. Agarwal R. Cell signaling and regulators of cell cycle as molecular targets for prostate cancer prevention by dietary agents. Biochem Pharmacol 2000;60:1051–9.
44. Wenzel U, Kunt S, Brendel MD, Daniel H. Dietary flavone is a potent apoptosis inducer in human colon carcinoma cells. Cancer Res 2000;60:3823–31.
45. Kamei, Hashimoto Y, Koide T, Kojima T, Hasegawa M. Anti-tumor effect of methanol extracts from red and white wines. Cancer Biother Radiopharm 1998;13:447–52.
46. Rabi T, Bishayee A. Terpenoids and breast cancer chemoprevention. Breast Cancer Res Treat 2009;115:223–39.
47. Singh MK, Khare G, Iyer SK, Shavan G, Tripathi Dk. Clerodendrum serratum: a clinical approach. J Appl Pharm Sci 2012;2:11-5.
48. Bhangare NK, Pansare TA, Ghongane BB, Nesari TM. Screening for the antifertility and antiallergic activity of Bhargi (Clerodendrum Serratum) in animals. Int J Pharmacol Biol Sci 2012;3:245-54.
49. Shi XF, Du DJ, Xie DC, Ran CQ. Studies on the antitumor effect of Clerodendrum bungei Steud or C. foetidum Bge. Pubmed 1993;18:687-90.
50. Kim SK, Cho SB, Moon HI. Anti-complement activity of isolated compounds from the roots of Clerodendrum bungeiSteud. Phytother Res 2010;24,1720-3.
51. Cheng HH, Wang HK, Ito J, Bastow KF, Tachibana Y, Nakanishi Y, et al. Cytotoxic pheophorbide-related compounds from Clerodendrum calamitosum and C. Cyrtophyllum. J Nat Prod 2001;64:915-9.
52. Sannigrahi S, Mazumder UK, Pal D, Mishra SL. Terpenoids of methanol extract of Clerodendrum infortunatum exhibit anticancer activity against Ehrlich's ascites carcinoma (EAC) in mice. Pharm Biol 2012;50:304-9.
53. Bhatnagar S, Pattanaik SR. Comparative analysis of cytotoxic and antioxidant activities of leaf and bark extracts of Clerodendrum viscosum and Clerodendrum phlomidis. Int J Biomed Adv Res 2012;3:385-90.
54. Islam MS, Mogha MMR, Ahamed SK, Ahmed J, Islam MA. A study on cytotoxic and anthelmintic activities of crude extract of leaves of Clerodendrum viscosum. Int Res J Pharm 2013;4:99-102.
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How to Cite
Haris, M., R. Mahmood, H. Rahman, and N. Rahman. “IN VITRO CYTOTOXIC ACTIVITY OF CLERODENDRUM INFORTUNATUM L. AGAINST T47D, PC-3, A549 AND HCT-116 HUMAN CANCER CELL LINES AND ITS PHYTOCHEMICAL SCREENING”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 1, Dec. 2015, pp. 439-44, https://innovareacademics.in/journals/index.php/ijpps/article/view/9644.
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