@article{Shasho_Sakur_Trefi_2018, title={SEPARATION AND ASSAY OF FOUR ANTIHISTAMINE DRUGS DIPHENHYDRAMINE, CHLORPHENIRAMINE, CYPROHEPTADINE AND FEXOFENADINE IN PHARMACEUTICAL FORMS BY A SINGLE HPLC METHOD}, volume={10}, url={https://journals.innovareacademics.in/index.php/ijpps/article/view/24819}, DOI={10.22159/ijpps.2018v10i4.24819}, abstractNote={<p><strong>Objective: </strong>The objective of this study was to develop and validate a single HPLC method, in order to separate and assay four antihistamine drugs diphenhydramine, chlorpheniramine, cyproheptadine and fexofenadine in pharmaceutical forms. This method was a practical additional choice in quality control laboratories.</p><p><strong>Methods: </strong>The chromatographic conditions comprised of a classical C<sub>18</sub>-type stationary phase (150 × 4.6 mm, 5μ), with a mobile phase consisting of, 2.5g of sodium octane sulfonic acid in a mixture of 500 ml of deionized water and 500 ml of acetonitrile, and apparent pH of 2.0 was adjusted with phosphoric acid. The flow rate was 1 ml/min; the detection wavelengths were at 220 nm, 230 nm, 265 nm and 254 nm for diphenhydramine, chlorpheniramine, cyproheptadine and fexofenadine respectively. The temperature was ambient temperature.</p><p><strong>Results: </strong>The method was validated for linearity with correlation coefficients very close to one, the accuracy with mean recovery values between 95.0-105.0%, precision with relative standard deviations of the calculated concentrations less than 5.0% and specificity in the presence of degradation products. Then it was used successfully to separate a mixture of them and to assay these drugs in pharmaceutical forms purchased from Syria.</p><p><strong>Conclusion: </strong>The results presented in this paper showed that the developed method was simple and applicable, for the separation and determination of the four drugs in their pharmaceutical forms.</p>}, number={4}, journal={International Journal of Pharmacy and Pharmaceutical Sciences}, author={Shasho, Hanan and Sakur, Amir Alhaj and Trefi, Saleh}, year={2018}, month={Apr.}, pages={53–60} }