@article{Subramaniyan_Dyavaiah_2018, title={EVALUATION OF ANTIOXIDANT ACTIVITY OF MAGNOLOL IN SACCHAROMYCES CEREVISIAE}, volume={10}, url={https://journals.innovareacademics.in/index.php/ijpps/article/view/25467}, DOI={10.22159/ijpps.2018v10i6.25467}, abstractNote={<p><strong>Objective: </strong>To investigate the antioxidant activity of magnolol in <em>Saccharomyces cerevisiae </em>(<em>S. cerevisiae</em>) model.</p><p><strong>Methods: </strong>Antioxidant activity of magnolol was demonstrated in <em>S. cerevisiae </em>using spot assay, colony forming unit (CFU) assay, detection of reactive oxygen species (ROS) by fluorescent microscopy and spectrofluorometer, lipid peroxidation (LPO) and catalase (CAT) assay.</p><p><strong>Results: </strong>A dose-dependent increase in sensitivity of <em>S. cerevisiae</em> was observed with increase in hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) concentration. At 1.5 mmol concentration of H<sub>2</sub>O<sub>2</sub>, we observed 50 % of cell survivability in CFU and spot assay results. The sensitivity of <em>S. cerevisiae </em>to H<sub>2</sub>O<sub>2</sub> was protected by magnolol treatment. In spot assay, magnolol pre-treatment showed the similar growth pattern as that of control and in CFU assay 75 % of survivability was observed. Fluorescence microscopic images and fluorescence intensity levels using 2´, 7´-dichlorodihydrofluoresceindiacetate (H<sub>2</sub>DCFDA) showed less number of fluorescence cells and 1.2 fold decrease in fluorescence intensity in magnolol pre-treated cells. The cellular protection in the cells pre–treated with magnolol followed by H<sub>2</sub>O<sub>2 </sub>was correlated with the decreased LPO and increase in CAT activity was recorded.</p><p><strong>Conclusion: </strong>The result of the present study demonstrates that magnolol protects <em>S. cerevisiae</em> cells from H<sub>2</sub>O<sub>2 </sub>induced oxidant mediated cell death.</p>}, number={6}, journal={International Journal of Pharmacy and Pharmaceutical Sciences}, author={Subramaniyan, Subasri and Dyavaiah, Madhu}, year={2018}, month={Jun.}, pages={104–107} }