@article{NURFITRIYANA_HARMITA_ISKANDARSYAH_2020, title={ANALYSIS OF LYNESTRENOL IN HUMAN PLASMA IN VITRO BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY UV-VIS: EUROPEAN MEDICINES AGENCY GUIDELINE}, volume={12}, url={https://journals.innovareacademics.in/index.php/ijpps/article/view/36763}, DOI={10.22159/ijpps.2020v12i5.36763}, abstractNote={<p><strong>Objective</strong>: Development and validation of reverse phase high performance liquid chromatographic (RP-HPLC) method with UV-Vis detector for <em>in vitro</em> determination of lynestrenol with levonorgestrel as an internal standard in human plasma.</p> <p><strong>Methods</strong>: The RP-HPLC method was developed using a C<sup>18 </sup>Sunfire<sup>© </sup>waters column with a mobile phase of acetonitrile containing 0.1% formic acid in water (60:40), respectively, at a flow rate of 1.0 ml/min and was detected at a wavelength of 204 nm. Lynestrenol and levonorgestrel were extracted from human plasma using pentane with protein precipitation method.</p> <p><strong>Results</strong>: The RP-HPLC method was able to selectively quantify lynestrenol in blood plasma on 40 ng/ml. The assay exhibited a linear dynamic range 40-1000 ng/ml for lynestrenol with retention time 4.0 second, and the coefficient correlation (r) was 0.9994. Accuracy (% diff) of this method was-10.81% to 8.72% with precision (CV) being 3.84% to 8.12%, and complete recovery was established to be 98.27% to 106.49%. The method was sensitive, selective, and has simple sample preparation extraction lynestrenol in plasma with pentane was successfully developed.</p> <p><strong>Conclusion</strong>: The method can be used to analyze lynestrenol in blood plasma, with a simple pretreatment procedure using pentane.</p>}, number={5}, journal={International Journal of Pharmacy and Pharmaceutical Sciences}, author={NURFITRIYANA, NURFITRIYANA and HARMITA, HARMITA and ISKANDARSYAH, ISKANDARSYAH}, year={2020}, month={May}, pages={80–84} }