@article{Saxena_Banerjee_Garg_Singh_Verma_Kushwaha_2015, title={ESBL, MBL AND AMP C-β LACTAMASES PRODUCED BY SUPERBUGS: AN EMERGING THREAT TO CLINICAL THERAPEUTICS}, volume={7}, url={https://journals.innovareacademics.in/index.php/ijpps/article/view/7313}, abstractNote={<p><strong>Objectives:</strong> The present study was undertaken to determine the prevalence of multi drug resistant (MDR) and multiple β-lactamase producing <em>Pseudomonas aeruginosa</em> isolates in lower respiratory tract infection (LRTI) patients at a tertiary care hospital in India.</p><p><strong>Methods:</strong> A total of 80 consecutive, non-duplicate isolates of <em>P. aeruginosa</em> were studied for the presence of class A or B β-lactamase. Antibiotic susceptibility tests and PCR amplification of genes encoding class A (PER-1 and CTX-M 1, 2, 9) and class B β-lactamases (<em>bla</em>VIM-2, <em>blaI</em>MP-1 and <em>bla</em>SIM-1) were performed.</p><p><strong>Results:</strong> Out of 80 <em>P. aeruginosa</em> isolates, 65% (52/80) of the isolates were MDR with 34 being Metallo-β-lactamase (MBL) producers, 23 were extended spectrum β-lactamase (ESBL) producers and 21 were positive for AmpC production. The cross-class resistance rates to other antibiotics was significantly higher in class A and B β-lactamase producers than in non-producers (P<0.05 for fluoroquinolone, aztreonam, ceftazidime and meropenem). Combined disk test (CDT) for MBL highest sensitivity and specificity compared to PCR. Combined disk method (CDM) for ESBL co-related well with PCR (sensitivity and specificity).</p><p><strong>Conclusion:</strong> This study reports the validation of a simple and accurate MBL and ESBL detection method which can be easily integrated into the daily routine of a clinical laboratory.</p><p> </p>}, number={9}, journal={International Journal of Pharmacy and Pharmaceutical Sciences}, author={Saxena, Shivani and Banerjee, Gopa and Garg, Rajiv and Singh, Mastan and Verma, S. K and Kushwaha, R. A. S.}, year={2015}, month={Sep.}, pages={353–356} }