TY - JOUR AU - Abu-raghif, Ahmed R. AU - Jasim, Ghaith A. AU - Hanoon, Muneer M. PY - 2017/02/01 Y2 - 2024/03/29 TI - ANTI-PROLIFERATIVE ACTIVITY OF ZIZYPHUS SPINA CHRISTI LEAVES METHANOL EXTRACT AGAINST RHABDOMYOSARCOMA (RD) CELL LINE JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 9 IS - 2 SE - Original Article(s) DO - 10.22159/ijpps.2017v9i2.15013 UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/15013 SP - 279-282 AB - <p><strong>Objective: </strong>The aim of this study was to investigate the capability of <em>Zizyphus spina christi</em> methanol extract to inhibit cancer cell line proliferation.</p><p><strong>Methods: </strong>The leaves of <em>Zizyphus spina christi</em> were extracted by cold maceration method. The anti-proliferative activity of the methanol extract against rhabdomyosarcoma (RD) cell line was tested by 3-(4, 5 Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemical constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was assessed by measuring free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. <strong></strong></p><p><strong>Results: </strong>The percentage extraction yield for leaves with methanol was 20.64%. The methanol extract showed dose dependent inhibition of RD cell line, the IC<sub>50</sub> was 154.44 µg/ml. GC-MS showed the presence of flavonoid fraction and other compounds with antioxidant activity. The methanol extract demonstrated DPPH scavenging activity with IC<sub>50</sub> of 33.91 mg/ml.</p><p><strong>Conclusion: </strong>Methanol extract showed<strong> </strong>potential anti-proliferative activity against RD cell line,<strong> </strong>which could be due to its antioxidant activity.</p> ER -