TY - JOUR AU - Saggoo, Manjit Inder Singh AU - ., Lovleen PY - 2017/10/02 Y2 - 2024/03/29 TI - SCREENING OF TOTAL PHENOL AND FLAVONOID CONTENT IN DIFFERENT CYTOTYPES OF TWO SPECIES OF ACHYRANTHES LINN. FROM WESTERN HIMALAYA, INDIA JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 9 IS - 10 SE - Original Article(s) DO - 10.22159/ijpps.2017v9i10.19780 UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/19780 SP - 205-210 AB - <p><strong>Objective: </strong>Genus <em>Achyranthes</em> Linn. belonging to family Amaranthaceae consists of six species. The present study was undertaken to screen the phenolic components in the different cytotypes of two species of Genus <em>Achyranthes</em> Linn. growing in western Himalaya, India.</p><p><strong>Methods: </strong>Methanol extract of leaves was used to determine the total phenol and flavonoid contents in different cytotypes of <em>A. aspera</em> Linn. and <em>A. bidentata </em>Blume by spectrophotometric method. Total phenol content was expressed as mg gallic acid g<sup>-1</sup>phenol and total flavonoid content as mg quercetin g<sup>-1</sup>flavonoid using the standard curves. Further, gallic acid content in methanol extracts of leaves was determined by RP-HPLC method using C-18 column, employing 0.01% (v/v) orthophosphoric acid: acetonitrile (98:2 v/v) as mobile phase at a flow rate of 1 ml/min with ultraviolet (UV) detection at 272 nm.</p><p><strong>Results: </strong>Hexaploid plants of <em>A. aspera</em> Linn. possess the higher amount of phenol (9.16±0.84 mg/g) and flavonoid (78.36±1.63 mg/g) constituents in the methanol extract of leaves as compared to its dodecaploid counterparts (7.86±0.08 mg/g and 70.20±1.81 mg/g respectively). Similarly, phenol and flavonoid content is found to be more in the methanol extract of leaves of hexaploid plants of <em>A. bidentata </em>Blume (11.93±0.59 mg/g and 115.92±1.32 mg/g respectively) as compared to its dodecaploid counterparts (9.46±0.75 mg/g and 107.76±0.94 mg/g respectively). Further, RP-HPLC analysis of gallic acid revealed that higher amount of gallic acid is present in dodecaploid plants of <em>A. aspera</em> Linn. (1.04±0.02 mg/g) and <em>A. bidentata </em>Blume (1.34±0.03 mg/g) as compared to hexaploid counterparts (1.01±0.01 mg/g and 1.22±0.05 mg/g respectively).</p><p><strong>Conclusion: </strong>The present investigation revealed that <em>A. aspera</em> Linn. and <em>A. bidentata </em>Blume<em> </em>plants show immense intraspecific variability in their phenolic components. Hence there is need to evaluate germplasm to select superior genotype for medicinal and conservation purpose.</p> ER -