TY - JOUR AU - T. R., Prashith Kekuda AU - L., Raghavendra H. AU - M., Shilpa AU - D., Pushpavathi AU - Petkar, Tejaswini AU - Siddiqha, Ayesha PY - 2017/10/02 Y2 - 2024/03/29 TI - ANTIMICROBIAL, ANTIRADICAL AND INSECTICIDAL ACTIVITY OF GARDENIA GUMMIFERA L. F. (RUBIACEAE) JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 9 IS - 10 SE - Original Article(s) DO - 10.22159/ijpps.2017v9i10.20252 UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/20252 SP - 265-272 AB - <p><strong>Objective: </strong>The present study was carried out to investigate antimicrobial, antiradical and insecticidal potential of leaf and fruit of <em>Gardenia gummifera</em> L. f. (Rubiaceae).</p><p><strong>Methods: </strong>The leaf and fruits were shade dried, powdered and extracted by maceration process using methanol. Antibacterial activity was evaluated against Gram positive and Gram negative bacteria by Agar well diffusion assay. Antifungal activity was determined against six seed-borne fungi by Poisoned food technique. Antiradical activity of leaf and fruit extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) radical scavenging assays. Insecticidal activity of leaf and fruit extracts, in terms of larvicidal and pupicidal activity, was assessed against larvae and pupae of <em>Aedes aegypti</em>.</p><p><strong>Results: </strong>Both the extracts inhibited all test bacteria. Marked antibacterial activity was displayed by fruit extract when compared to leaf extract. <em>S. epidermidis</em> and <em>E. coli</em> were inhibited to highest and least extent by both extracts respectively. Fruit extract was found to exhibit higher antifungal effect when compared to leaf extract. Leaf extract and fruit extract exhibited highest inhibitory activity against <em>A. niger</em> and <em>A. flavus</em> respectively. Leaf and fruit extracts scavenged DPPH radical's dose dependently with an IC<sub>50</sub> value of 49.01µg/ml and 2.53µg/ml respectively. The scavenging of ABTS by leaf and fruit extracts was dose dependent and the IC<sub>50</sub> value for leaf and fruit extract was 2.58µg/ml and 2.31µg/ml respectively. Fruit extract was shown to exhibit marked antiradical activity when compared to leaf extract. Leaf and fruit extracts exhibited dose dependent insecticidal activity in terms of larvicidal and pupicidal activity and the susceptibility of larvae and pupae to extracts was in the order II instar larvae&gt;IV instar larvae&gt;pupae. Fruit extract displayed marked insecticidal potential when compared to leaf extract.</p><p><strong>Conclusion: </strong>Overall, fruit extract of <em>G. gummifera</em> exhibited marked antimicrobial, antiradical and insecticidal activity when compared to leaf extract. The plant can be used for developing agents/formulations effective against infectious microorganisms, oxidative stress and insect vectors that transmit dreadful diseases. The observed bioactivities could be ascribed to the presence of active principles which are to be isolated and characterized.</p> ER -