TY - JOUR AU - Athithan, Velan AU - Ramesh, Ramasamy AU - Srikumar, Kotteazeth PY - 2018/02/01 Y2 - 2024/03/29 TI - 28-HOMOCASTASTERONE: A NOVEL DIETARY PHYTO KETO OXYSTEROL MODULATING TESTICULAR STEROID METABOLISM AND LxR mRNA EXPRESSION IN DIABETIC RAT JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 10 IS - 2 SE - Original Article(s) DO - 10.22159/ijpps.2018v10i2.23779 UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/23779 SP - 162-167 AB - <p><strong>Objective: </strong>Present study aims to investigate 28-homocastasterone (28-HC) influences on testicular tissue in the normal and diabetic rat.</p><p><strong>Methods: </strong>Induction of diabetes was achieved by single peritoneal injection of streptozotocin (60 mg/kg b. wt) followed by 28-HC (100 µg/150 gm body weight) administration by oral gavage for 15 consecutive days to experimental rats. 3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase activities, testosterone level, Liver X Receptor (LxR) mRNA expression, malondialdehyde (MDA), reduced glutathione (GSH) and testis histology was analysed.</p><p><strong>Results: </strong>Increased cholesterol level, 3β-hydroxysteroid dehydrogenase (3βHSD), 17β-hydroxysteroid dehydrogenase (17βHSD) activities, testosterone level with significant elevation of LxR-α and β mRNA expression in treated rat testis. A significant reduction found inMDA and increased reducedGSH along with improved testicular architecture was observed.</p><p><strong>Conclusion: </strong>In the present study demonstrated that 28-HC induced 3βHSD, 17βHSD enzyme activity and testosterone level, thus indicative of steroidogenic potential and capable of transactivating LxR-α and β molecular operative in rat testicular tissue.</p> ER -