TY - JOUR AU - Houchi, Selma AU - Mahdadi, Rachid AU - Messasma, Zakia AU - Sahli, Farida PY - 2014/09/01 Y2 - 2024/03/29 TI - THE INHIBITORY EFFECT OF METHANOLIC AND AQUEOUS EXTRACTS OF TERMINALIA CHEBULA DRIED FRUITS ON METALLOBETALACTAMASES OF ACINETOBACTER BAUMANNII JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 6 IS - 9 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/2484 SP - 579-582 AB - <p><strong>Objective: </strong>toevaluate Metallo-betalactamase (MBL) inhibitory effect and antimicrobial activity against <em>Acinetobacter baumannii </em>of methanolic and aqueous extracts of <em>Terminaliachebula</em>dried fruits.<strong></strong></p><p><strong>Methods: </strong>effect of EDTA (Ethylenediaminetetraacetic acid), methanolic and aqueous extracts of dried fruits of <em>T. chebula</em> on Metallo-betalactamase activity was studied <em>in vitro</em>by using cephaloridin asasubstrate. The degree of inhibition was determined byIC<sub>50</sub>and the antimicrobial activities of both extracts against <em>Acinetobacter baumannii </em>were qualitatively and quantitatively assessed by the presence or absence of inhibition zones, MIC and MBC values.</p><p><strong>Results: </strong>EDTA, methanolic and aqueous extracts inhibit metallo-betalactamases activity withIC<sub>50</sub>(3.98 mM, 18.45 and 19.68µg/ml), respectively. Polyphenols in aqueousand methanolic extracts are 513.094±3.061 and 496±2.856 µg GAE/mg, respectively.</p><p><strong>Conclusion: </strong>These results indicate that both extracts of <em>T. chebula</em> are rich in polyphenols and have a strong inhibitory effect on metallo-betalactamases in a competitive manner compared to EDTA. In addition, both extracts of <em>T. chebula </em>were also screened for their remarkable antibacterial and bactericidal activity against <em>Acinetobacter baumannii </em>confirmed by low minimum inhibitory and bactericidal concentration (MIC and MBC).</p><p> </p> ER -