TY - JOUR AU - Mandal, Meghamala AU - Das, Koushik AU - Nandi, Dilip Kumar AU - Das, Balaram AU - J., Sreenivas R. PY - 2019/03/01 Y2 - 2024/03/28 TI - ANALYSIS OF FATTY ACIDS FROM OIL OF GREEN TEA (CAMELLIA SINENSIS L) BY GAS CHROMATOGRAPHY COUPLED WITH FLAME IONIZATION DETECTOR AND ITS ANTICANCER AND ANTIBACTERIAL ACTIVITY IN VITRO JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 11 IS - 3 SE - Original Article(s) DO - 10.22159/ijpps.2019v11i3.29888 UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/29888 SP - 104-111 AB - <p><strong>Objective: </strong>Tea is a widely consumed beverage worldwide. The effect of green tea is mainly due to its high polyphenols-(-) epigallocatechin-3-gallate (EGCG) content in the culture of cancer cell and bacterial cells. The present work was carried out to investigate the efficacy of green tea oil (GTO) against cancer cells and bacterial cells.</p><p><strong>Methods: </strong>In this study green tea oil was prepared from green tea for different experiment and determination of fatty acids profile from green tea oil. In the present study, peripheral blood lymphocyte (PBL) was chosen as human peripheral blood lymphocytes and blood cancer MCF-7 cells were chosen as human cancer cells. To fulfill our aims and also to evaluate the activity of this phytomedicine against normal lymphocytes and cancer cells the cell samples were divided into 26 experimental groups in the following ways. Each Petri dish contains 2 X 10<sup>5</sup> cells.</p><p><strong>Results: </strong>GTO shows a potent anticancer agent but nontoxic to normal cells. The GTO decreases the reduced glutathione (GSH) level and increase the oxidized glutathione (GSSG) level significantly (P&lt;0.05) in MCF-7 cells. But in lymphocytes the GSH level and GSSG level were almost the same with the control group but doxorubicin (DOX) significantly decreased the GSH and increase the GSSG level. Green tea oil treatment causes generation of reactive oxygen species (ROS) in MCF-7 cells revealed by DCFH<sub>2</sub>DA staining. Agar diffusion test shows the GTO is effective against multi-drug resistant bacteria.</p><p><strong>Conclusion: </strong>This phytomedicine has a potent anticancer activity without damaging the normal lymphocytes. So, this drug can be used for further treatment of anticancer and antibacterial.</p> ER -