TY - JOUR AU - K R, Krishna Kumar AU - P, Jayaprakash A AU - K, Srinivasan K AU - Harindran, Jyoti PY - 2015/02/01 Y2 - 2024/03/29 TI - EXTRACTION, ISOLATION AND IDENTIFICATION OF PHYTOCONSTITUENTS PRESENT IN THE HEMIONITIS ARIFOLIA LEAVES JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 7 IS - 2 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/3780 SP - 477-484 AB - <div class="WordSection1"><p><strong>Objective: </strong>The investigation was carried out to prepare 95 and 50% ethanol extract of <em>Hemionitis arifolia</em> leaves, and for the identification and quantification of the phytoconstituents present in it.</p><p><strong>Methods: </strong>Soxhlet extraction method was followed to prepare the extracts, followed by standard phytochemical methods to identify the phytoconstituents present in it. Paper chromatography, HPLC, LC MS/MS and LCMS studies were then done for the confirmation, quantification of phytoconstituents and detection of mass values of other unidentified phytoconstituents present in the extract.</p><p><strong>Results: </strong>Extractive values were found to be 14.6 and 15.6% w/w respectively. In the phytochemical studies, presence of carbohydrates, glycosides, saponins, flavonoids, phenolics, tannins, phytosterols and fixed oils was identified. On doing paper chromatography with Whatman No.1 filter paper using 15% acetic acid solvent system, six different spots with yellow-orange colour were detected under UV visualization. Out of the six spots, four were identified as quercetin, kaempferol, apigenin and rutin by co-chromatography. In the RPHPLC analysis, rutin, quercetin and kaempferol were identified and quantified. In the LC MS/MS analysis, rutin, apigenin and kaempferol were identified and quantified. In the LCMS analysis, ten different m/z values were found, among which m/z 609 was identified as rutin.</p><p><strong>Conclusion</strong>:<strong> </strong>The presence of various flavonoids and related glycosides is indicative of potential for medicinal use of this plant.</p><p> </p></div> ER -