TY - JOUR AU - P., Rout S. AU - M., Kar D. AU - E., Jagadeeshreddy AU - S., Manoj AU - T, Sengottuvel PY - 2015/02/01 Y2 - 2024/03/28 TI - IN VITRO ANTIOXIDANT PROPERTY OF FRACTIONS OF ANNONA RETICULATA (L) LEAVE JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 7 IS - 2 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/4138 SP - 299-303 AB - <p><strong>Objective: </strong>The present study has been carried out to evaluate the antioxidant property of different fractions prepared from hydro-alcoholic extract of <em>Annona reticulata</em> L. leave. Effort also has been made to estimate the flavonoid and phenolics content of the fractions.</p><p><strong>Method: </strong>The antioxidant activity has been studied <em>in vitro</em> by using Nitric oxide-scavenging assay, Free radical scavenging activity in DPPH assay and Iron chelating activity assay.</p><p><strong>Results: </strong>In Nitric oxide-scavenging assay the IC<sub>50</sub> values for Ethylacetate fraction, methanol fraction and residue/aqueous fraction are 476.43 µg/mL, 328.09 µg/mL and 329.61 µg/mL respectively. In DPPH assay the IC<sub>50</sub> values for Ethylacetate fraction, methanol fraction and residue/aqueous fraction are 87.50 µg/mL, 161.06 µg/mL and 90.36 µg/mLrespectively. The IC<sub>50</sub> values of the Ethylacetate fraction, methanol fraction and residue/aqueous fraction in Iron chelating assay are 210.86 µg/mL, 462.38 µg/mL and 586.14 µg/mL respectively. The flavonoid content of Ethylacetate fraction, methanol fraction and residue/aqueous fraction are 8.73, 7.45 and 8.62 respectively expressed as mg/g of Catechol. Similarly the phenol content of Ethylacetate fraction, methanol fraction and residue/aqueous fraction are 8.43, 7.25 and 6.23 respectively expressed as mg/g of Catechol.</p><p><strong>Conclusion: </strong>The results suggest that all the tested fractions are having antioxidant property, but the ethhylacetate fraction is having significantly higher flavonoid and phenol content. Due to presence of higher flavonoid and phenol content in ethylacetate fraction, it may be considered as the fraction with better pharmacological property in comparison to other tested fractions.<strong></strong></p><p class="Default"> </p> ER -