TY - JOUR AU - SULTANA, SIRIN SALMA PY - 2021/09/01 Y2 - 2024/03/29 TI - IN VITRO ANTILEISHMANIAL ACTIVITIES OF THREE MEDICINAL PLANTS: ARGEMONE MEXICANA, MURRAYA KOENIGII AND CINNAMOMUM TAMALA AGAINST MILTEFOSINE RESISTANT PROMASTIGOTES OF LEISHMANIA DONOVANI PARASITES JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 13 IS - 9 SE - Original Article(s) DO - 10.22159/ijpps.2021v13i9.42349 UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/42349 SP - 27-33 AB - <p><strong>Objective: </strong>Leishmaniasis is one of the neglected tropical diseases in terms of drug development and discovery. Non-responsiveness and resistance to the drug in <em>Leishmania</em> species need to develop new antileishmanial potentials; herbal medicines could be the alternative one.</p><p><strong>Methods: </strong>In the present study, semi-purified fractions were prepared from the traditionally used three medicinal plants of India: <em>Argemone mexicana </em>(aerial shoot), <em>Murraya koenigii </em>(stem), and <em>Cinnamomum tamala </em>(bark) by using multiple solvent systems (non-polar to polar, beginning with petroleum ether followed by <em>n</em>-hexane, benzene, and chloroform) and an effort was given to assess the leishmanicidal activities against <em>Leishmania donovani </em>miltefosine resistant HePC-R (Ld/MIL-30) promastigotes <em>in vitro </em>and the IC50 concentrations were estimated.</p><p><strong>Results: </strong>The study revealed that the semi-purified fractions of <em>A. mexicana, M. koenigii, </em>and <em>C.</em> <em>tamala </em>have effective antileishmanial activities and the 50% inhibitory concentrations (IC50) are 50 μg/ml, 98 μg/ml, and 200 μg/ml, respectively. At these (IC50) concentrations, these plant semi-purified fractions were found to interfere in lipid and protein biosynthesis, alter cell morphology, DNA content, mitochondrial membrane potential, generating ROS, and apoptosis in promastigotes. The semi-purified fractions were also found noticeably non-toxic towards host splenocytes.</p><p><strong>Conclusion: </strong>These results could suggest that <em>A. mexicana, M. koenigii, </em>and <em>C.</em> <em>tamala </em>could carry potential novel compounds for the development of new drugs against Leishmaniasis.</p> ER -