TY - JOUR AU - Pradhan, Nayan AU - Rajkhowa, Hiyashree AU - Giri, Hemant AU - Shrestha, Bhupendra PY - 2015/11/01 Y2 - 2024/03/29 TI - SIMULTANEOUS SPECTROPHOTOMETRIC ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DOXORUBICIN HYDROCHLORIDE JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 7 IS - 11 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/4387 SP - 21-26 AB - <p><strong>Objective:&nbsp;</strong>To develop a simple, accurate, and precise spectrophotometric method for the simultaneous estimation of moxifloxacin hydrochloride (MOX) and doxorubicin hydrochloride (DXR).</p><p><strong>Methods:&nbsp;</strong>MOX and DXR solution were simultaneously determined in 0.1M HCl at their respective&nbsp;<em>λ</em><sub>max</sub>. The absorbance&nbsp;<em>λ</em><sub>max</sub>&nbsp;of MOX and DXR was 295 nm and 480 nm, respectively. The developed method was validated according to ICH guidelines for parameters like linearity, accuracy, precision, ruggedness and robustness.</p><p><strong>Results:&nbsp;</strong>Molar absorptivities of MOX and DXR were found to be more in 0.1M HCl with compared to water, methanol and 0.1M NaOH. Linearity was obtained over the range 0.5–20.0 μg/ml and 1.5-50 μg/ml with a lower limit of quantitation of 0.25 μg/ml and 1.5 μg/ml for MOX and DXR, respectively. For each level of samples, inter-and intra-day precision ( % RSD) was&lt;1.3 % and&lt;1.4 % for MOX and&lt;2.5 and 2.4 % for DXR, respectively. The mean recovery of MOX and DXR was in the range 96.21 %-98.77 % and 97.13 %-99.64 %, respectively.</p><p><strong>Conclusion:&nbsp;</strong>The method developed was validated as per ICH guidelines for parameters like linearity, accuracy, method precision, robustness and ruggedness. The results obtained were well within the acceptable criteria. The method can be used for routine analysis of MOX and DXR.</p> ER -