TY - JOUR AU - Gopinathan, K. Gomathi AU - Gayathri, D. PY - 2015/02/01 Y2 - 2024/03/19 TI - PROTECTIVE EFFECTS OF Α - CRYSTALLIN ON Β - AMYLOID (Aβ) INDUCED TOXICITY JF - International Journal of Pharmacy and Pharmaceutical Sciences JA - Int J Pharm Pharm Sci VL - 7 IS - 2 SE - Erratum DO - UR - https://journals.innovareacademics.in/index.php/ijpps/article/view/4979 SP - 588 AB - <p><strong>Objectives:</strong> To investigate the protective role of α-crystallin against β-amyloid aggregation.</p><p><strong>Methods:</strong> <em>In vitro</em> spectroscopic methods and cell culture studies were done to validate our objective.</p><p><strong>Results:</strong> The molecular basis of alzhemiers disease has been proposed to be accumulation and aggregation of β-amyloid (Aβ). However, prevention of β-amyloid aggregation is still a promising means to reduce its neurotoxicity. In this work, we show that α-crystallin was able to inhibit cellular toxicity of Aβ on astrocytes and lymphocytes. Theα−crystallin (αA and αB): the two vertebrate eye lens proteins that are related to the small heat shock protein family, was able to reverse the oxidative stress induced by Aβ1-42. Treatment of α-crystallin enhances the activity of proteasome and it also induces the expression of Hsp70 which is known to inhibit the intramolecular misfolding. We also demonstrate that Aβ1-42 suppresses the expression of TriC chaperonin subunits TCPβ and TCPε, which are known to play a role in folding of misfolded proteins.α-crystallin reverses this effect and enhances the expression of TCPβ and TCPε.</p><p><strong>Conclusions:</strong> Research findings in this study provide the basis for the development of novel pharmacotherapy for Alzhemier's disease.</p> ER -